MSH2 and MSH6 in mismatch repair system account for soybean (Glycine max (L.) Merr.) tolerance to cadmium toxicity by determining DNA damage response(1区SCI).
MSH2 and MSH6 in mismatch repair system account for soybean (Glycine max (L.) Merr.) tolerance to cadmium toxicity by determining DNA damage response(1区SCI).
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DOI：
10.1021/acs.jafc.9b06599
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
Wan Liu
中科院分区：
文献类型：
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作者：
Qiang Zhao;Hetong Wang Yanli Du Hilary J. Rogers;Zhixin Wu Sen Jia Futi Xie;Wan Liu
我们的目的是研究调节两个大豆品种镉耐受性的 DNA 错配修复 (MMR) 基因。品种辽豆 10（LD10，镉敏感）和神农 20（SN20，耐镉）幼苗在含有 0–2.5 mg·L–1Cd 的 Murashige 和 Skoog (MS) 培养基上水培 4 天。镉胁迫在 LD10 中诱导的随机扩增多态性 DNA (RAPD) 多态性少于 SN20 根中的随机扩增多态性 DNA (RAPD) 多态性，导致 LD10 中的 G1/S 停滞和 SN20 根中的 G2/M 停滞。 LD10-TRV-MLH1 植株中 MLH1 的病毒诱导基因沉默 (VIGS) 显示 G1/S 停滞显着减少，但在 Cd 胁迫下根长/面积增加。然而，在 SN20-TRV-MSH2 和 SN20-TRV-MSH6 植株中，G1/S 停滞增加，G2/M 停滞减少，在 Cd 胁迫下，根长/面积减少。综上所述，我们得出结论，参与 G2/M 停滞的 MSH2 和 MSH6 的低表达导致 Cd 诱导的 DNA 损伤识别绕过 MMR 系统，在 MLH1 的帮助下激活 G1/S 停滞。这导致 LD10 根部生长受到抑制，解释了大豆镉耐受性的品种间差异。
Our aim was to investigate DNA mismatch repair (MMR) genes regulating cadmium tolerance in two soybean cultivars. Cultivars Liaodou 10 (LD10, Cd-sensitive) and Shennong 20 (SN20, Cd-tolerant) seedlings were grown hydroponically on Murashige and Skoog (MS) media containing 0–2.5 mg·L–1Cd for 4 days. Cd stress induced less random amplified polymorphism DNA (RAPD) polymorphism in LD10 than in SN20 roots, causing G1/S arrest in LD10 and G2/M arrest in SN20 roots. Virus-induced gene silencing (VIGS) ofMLH1in LD10-TRV-MLH1plantlets showed markedly diminished G1/S arrest but enhanced root length/area under Cd stress. However, an increase in G1/S arrest and reduction of G2/M arrest occurred in SN20-TRV-MSH2and SN20-TRV-MSH6plantlets with decreased root length/area under Cd stress. Taken together, we conclude that the low expression ofMSH2andMSH6, involved in the G2/M arrest, results in Cd-induced DNA damage recognition bypassing the MMR system to activate G1/S arrest with the assistance of MLH1. This then leads to repressed root growth in LD10, explaining the intervarietal difference in Cd tolerance in soybean.