Rapid identification of red-flesh loquat cultivars using EST-SSR markers based on manual cultivar identification diagram strategy.
Rapid identification of red-flesh loquat cultivars using EST-SSR markers based on manual cultivar identification diagram strategy.
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DOI：
10.4238/2014.april.29.17
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发表时间：
2014-04
期刊：
影响因子：
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通讯作者：
X. Y. Li;H. X. Xu;J. W. Chen
中科院分区：
文献类型：
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作者：
X. Y. Li;H. X. Xu;J. W. Chen
人工品种鉴定图是一种基于DNA标记的植物品种鉴定新策略，为有效分离品种提供了信息。用25对苹果EST-SSR引物对不同栽培品种的供试材料进行了扩增。这些EST-SSR引物对不同品种的扩增产物清晰，对供试材料的转移率较高，达84%；11对EST-SSR引物扩增出多态性产物。通过对24份红肉莲子材料的分析，发现只有7对引物能将它们全部清晰地分离出来。利用DNA指纹图谱和EST-SSR引物的多态性条带构建了24个品种的品种鉴定图。根据品种间的多态性条带，可以将24个品种中的任意两个品种快速分离，并在品种鉴定图上的正确位置标记了相应的引物。该品种鉴定图可将24个红肉品种区分开来，为品种鉴定、种质资源管理和育种工作提供了依据。
Manual cultivar identification diagram is a new strategy for plant cultivar identification based on DNA markers, providing information to efficiently separate cultivars. We tested 25 pairs of apple EST-SSR primers for amplification of PCR products from loquat cultivars. These EST-SSR primers provided clear amplification products from the loquat cultivars, with a relatively high transferability rate of 84% to loquat; 11 pairs of primers amplified polymorphic products. After analysis of 24 red-fleshed loquat accessions, we found that only 7 pairs of primers could clearly separate all of them. A cultivar identification diagram of the 24 cultivars was constructed using polymorphic bands from the DNA fingerprints and EST-SSR primers. Any two of the 24 cultivars could be rapidly separated from each other, according to the polymorphic bands from the cultivars; the corresponding primers were marked in the correct position on the cultivar identification diagram. This red-flesh loquat cultivar identification diagram can separate the 24 red-flesh loquat cultivars, which is of benefit for loquat cultivar identification for germplasm management and breeding programs.