Expression and secretion of immunoglobulin alpha heavy chain with diverse VDJ recombinations by human epithelial cancer cells
Expression and secretion of immunoglobulin alpha heavy chain with diverse VDJ recombinations by human epithelial cancer cells
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DOI：
10.1016/j.molimm.2006.11.010
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发表时间：
2007-03-01
影响因子：
3.6
通讯作者：
Cao, Ya
中科院分区：
文献类型：
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作者：
Zheng, Hui;Li, Ming;Cao, Ya
一般来说，只有B淋巴细胞表达免疫球蛋白。最近，我们意外地在人上皮癌细胞中发现了Ig α重链的表达。我们首次在多种癌细胞系中检测到Ig VDJ-C α和I α - c α转录本。此外，我们还分析了人类癌细胞中Ig重链基因组位点的结构。我们发现癌细胞有重组的VDJ区，但在种系构型中有Ig S α区，这与B细胞中的Ig表达模式不同。而人上皮细胞肿瘤具有ag -1和ag -2等必需效应物，但不具有激活诱导胞苷脱氨酶（AID）蛋白。这为Ig表达式提供了进一步的证明。此外，我们发现人类癌细胞不仅表达Ig α链蛋白，还以分泌IgA （SIgA）模式分泌该蛋白。重要的是，在不同上皮来源的人类癌细胞中发现了不同的CDR3重组。由于IgA是参与粘膜局部免疫的关键免疫球蛋白，因此，Ig α重链的异常表达可能有助于我们进一步了解肿瘤的发生和免疫。(c) 2006 Elsevier Ltd.版权所有。
Generally, only B lymphocytes express immunoglobulin. Recently, we found the expression of Ig alpha heavy chain in human epithelial cancer cells unexpectedly. We first detected Ig VDJ-C alpha and I alpha-C alpha transcripts in multiple cancer cell lines. Further, the configuration of the Ig heavy chain genomic locus was analyzed in human cancer cells. We found that cancer cells have the recombination VDJ region, but bear Ig S alpha region in germline configuration, which is different from Ig expression pattern in B cells. And human epithelial cancers possess the essential effectors including RAG-1 and RAG-2, but not activation induced cytidine deaminase (AID) protein. These provide further proofs for Ig alpha expression. In addition, we found that human cancer cells not only express the protein of Ig alpha chain, but also secrete the protein in secretory IgA (SIgA) pattern. Importantly, diverse CDR3 recombinations were found in human cancer cells of different epithelial origin. Since IgA is the key immunoglobulin which contributes to local immunity of mucous membrane, the aberrant expression of Ig alpha heavy chain might increase our further comprehension to development and immunity of cancers. (c) 2006 Elsevier Ltd. All rights reserved.