Ethanol Inhibition of Thrombus Formation on Collagen-Coated Glass
Ethanol Inhibition of Thrombus Formation on Collagen-Coated Glass
复制标题
乙醇对胶原涂层玻璃上血栓形成的抑制作用
作者：
C. Owens;L. Mclntire;A. Lasslo
应用荧光视频显微镜观察乙醇对血小板壁血栓形成的影响。用乙醇处理的全血在平行板流室中以1000 /s的剪切速率灌注到胶原蛋白涂层玻璃上。采用数字图像处理和光电二极管测量来分析该表面上血栓生长的动态。0.02% v/v的乙醇浓度可抑制45 + 33%（±sd）的正常血小板在载玻片上的聚集，而0.2% v/v的乙醇可抑制82±15%的壁血栓形成。当乙醇浓度达到0.1% v/v时，血小板对胶原表面的粘附不受影响，而0.2% v/v的乙醇对粘附和聚集都有影响。这些结果表明，低浓度的乙醇能以生理剪切速率抑制受损血管模型壁上血栓的形成。在测量大块聚集的系统中，例如在聚集测定中，不会检测到这种抑制作用。
Summary Epi-fluorescent video microscopy was used to evaluate the effect of ethanol on platelet mural thrombus formation. Whole blood, treated with ethanol, was perfused over collagen coated glass in a parallel-plate flow chamber at a shear rate of 1,000/s. Digital image processing and photodiode measurements were used to analyze the dynamics of thrombus growth on this surface. Ethanol concentrations as low as 0.02% v/v were found to inhibit 45 + 33% (± S.D.) of normal platelet accumulation on the slide while 0.2% v/v ethanol effected an 82 ± 15% inhibition of mural thrombus formation. While platelet adhesion to the collagen surface appeared unaffected by ethanol concentrations up to 0.1% v/v, 0.2% v/v ethanol had an effect on adhesion as well as aggregation. These results imply that low ethanol concentrations inhibit the formation of mural thrombi in a model of a damaged blood vessel at physiological shear rates. This inhibition would not be detected in systems which measure bulk aggregation, e.g. in aggregometric determinations.
登录
查看更多内容
乙醇通过激活磷酸肌醇特异性磷脂酶 C 来刺激人血小板的形状变化。
影响因子：
3.9
作者：
Rubin,R;Ponnappa,BC;Thomas,AP;Hoek,JB
通讯作者：
Hoek,JB
凝血酶原酶复合物的组装和表达中的脂质流动性。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Higgins,DL;Callahan,PJ;Prendergast,FG;Nesheim,ME;Mann,KG
通讯作者：
Mann,KG
聚集抑制剂的化学组成与人血小板反应谱之间的关系。
DOI：
10.1016/0005-2736(84)90520-0
发表时间：
1984
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Lasslo,A;Quintana,RP;Johnson,RW;Naylor,JL;Dugdale,M
通讯作者：
Dugdale,M
凝血酶原酶复合体组装。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Krishnaswamy,S;Jones,KC;Mann,KG
通讯作者：
Mann,KG
长期摄入乙醇会增加肝微粒体中钙的吸收和对乙醇分子紊乱的抵抗力。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Ponnappa,BC;Waring,AJ;Hoek,JB;Rottenberg,H;Rubin,E
通讯作者：
Rubin,E