Transcriptional activity of GLI1 is negatively regulated by protein kinase A
Transcriptional activity of GLI1 is negatively regulated by protein kinase A
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DOI：
10.1515/bc.2000.070
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发表时间：
2000-07-01
影响因子：
3.7
通讯作者：
Rüther, U
中科院分区：
文献类型：
--
作者：
Kaesler, S;Lüscher, B;Rüther, U
转录因子GLI 1是GLI家族的三个脊椎动物成员之一，其特征在于高度保守的五个锌指的DNA结合结构域。我们分析了人类GLI 1是否是PKA调控的靶点。研究发现PKA抑制GLI 1的转录活性。然而，没有证据表明蛋白水解加工或改变GLI 1的亚细胞分布。反应性PKA位点（aa 333 -336）定位于GLI 1的第二锌指。丝氨酸336位的突变显示PKA也可以刺激GLI 1的转录活性。因此，我们的数据表明PKA对人GLI 1的负调节和正调节。
The transcription factor GLI1 is one of three vertebrate members of the GLI family, which is characterized by a highly conserved DNA-binding domain of five zinc fingers. We have analyzed whether human GLI1 is a target of PKA regulation. It was found that PKA inhibits GLI1 transcriptional activity. However, no evidence for proteolytic processing or for alteration in the subcellular distribution of GLI1 was obtained. The responsive PKA site (aa333-336) was localized to the second zinc finger of GLI1. Mutation of Ser336 revealed that PKA could also stimulate GLI1 transcriptional activity. Thus, our data demonstrate both negative and positive regulation of human GLI1 by PKA.