Activation of inflammatory mediators in rat renal isografts by donor brain death
Activation of inflammatory mediators in rat renal isografts by donor brain death
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DOI：
10.1097/00007890-200002150-00017
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发表时间：
2000-02-15
期刊：
影响因子：
6.2
通讯作者：
Tilney, NL
中科院分区：
文献类型：
--
作者：
Kusaka, M;Pratschke, J;Tilney, NL
背景。脑死亡（BD）被认为通过触发一系列非特异性炎症事件影响移植器官的早期过程，这些炎症事件反过来可能增加免疫宿主反应的动力学和强度。本研究比较了ED供者肾脏同种移植物与正常麻醉、通气对照组肾脏同种移植物的早期非特异性、细胞和分子变化。方法：脑死亡诱导后，机械通气6小时，摘除器官。只纳入血压稳定的大鼠（平均动脉压bbb80 mmHg）。每日测定血清肌酐。代表性移植物：在脑死亡后6小时和移植物植入后1小时至5天采集形态学、免疫组织学和逆转录-聚合酶链反应。采用酶联免疫吸附法检测血清细胞因子的存在。接受ED供体的患者血清肌酐水平略有上升。血清白细胞介素-1 β水平在6小时内较对照组升高(P
Background. Brain death (BD) has been thought to influence the early course of transplanted organs by triggering a series of nonspecific inflammatory events that in turn may increase the kinetics and intensity of the immunological host responses. In this study early nonspecific, cellular, and molecular changes occurring in kidney isografts from ED donors are compared with those from normal anesthetized, ventilated controls.Methods, After induction of brain death, the animals were mechanically ventilated for 6 hr before organ removal. Only rats with stable blood pressure (mean arterial pressure >80 mmHg) were included. Serum creatinines were measured daily. Representative grafts were: harvested 6 hr after brain death and between 1 hr and 5 days after engraftment for morphology, immunohistology, and reverse transcriptase-polymerase chain reaction. The presence of serum cytokines was assessed by enzyme linked immunoabsorbant assay.Results. Serum creatinine levels rose slightly in recipients from ED donors. Serum interleukin-1 beta levels increased within 6 hr versus controls (P