Preparative Separation of Monoterpenoid Indole Alkaloid Epimers from Ervatamia yunnanensis Tsiang by pH-Zone-Refining Counter-Current Chromatography Combined with Preparative High-Performance Liquid Chromatography
Preparative Separation of Monoterpenoid Indole Alkaloid Epimers from Ervatamia yunnanensis Tsiang by pH-Zone-Refining Counter-Current Chromatography Combined with Preparative High-Performance Liquid Chromatography
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pH 区精制逆流色谱结合制备型高效液相色谱制备云南麻药中单萜吲哚生物碱差向异构体
DOI：
10.3390/molecules24071316
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发表时间：
2019
期刊：
影响因子：
4.6
通讯作者：
Feng Jin Hong
中科院分区：
文献类型：
--
作者：
Zhou Jie;Du Si Yu;Dong Hong Jing;Fang Lei;Feng Jin Hong
建立了一种有效的分离纯化云南狗牙花单萜吲哚生物碱差向异构体的方法。利用该方法，从2.1g E. L.粗提物中成功地纯化了两对MIA差向异构体，包括麦角胺（72 mg，1）、20-表-麦角胺（27 mg，4）、dregamine（95 mg，2）、tabernaemontanine（129 mg，3），沿着两种MIA，apparicine（112 mg，5）和isovoacangine（15 mg，6）。经HPLC测定，纯度均在95%以上。MIA的结构通过ESI-MS，1D和2D NMR进行了鉴定。
An effective method was developed for the preparative separation and purification of monoterpenoid indole alkaloid epimers from Ervatamia yunnanensis Tsiang using a combination of pH-zone-refining counter-current chromatography and preparative high-performance liquid chromatography. With this method, two pairs of MIA epimers including ervatamine (72 mg, 1), 20-epi-ervatamine (27 mg, 4), dregamine (95 mg, 2), tabernaemontanine (129 mg, 3), along with two MIAs, apparicine (112 mg, 5) and isovoacangine (15 mg, 6), were successfully purified from 2.1 g crude extract of E. yunnanensis, each with a purity of over 95% as determined by HPLC. The structures of the MIAs were identified by ESI-MS, 1D, and 2D NMR.