Interaction of amphiphiles with integral membrane proteins. I. Structural destabilization of the anion transport protein of the erythrocyte membrane by fatty acids, fatty alcohols, and fatty amines.
Interaction of amphiphiles with integral membrane proteins. I. Structural destabilization of the anion transport protein of the erythrocyte membrane by fatty acids, fatty alcohols, and fatty amines.
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两亲物与完整膜蛋白的相互作用。
DOI：
10.1016/0005-2736(88)90512-3
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Low,PS
中科院分区：
文献类型：
--
作者：
Gruber,HJ;Low,PS
用差示扫描量热法研究了两亲模型化合物对人红细胞膜阴离子交换蛋白（带3）结构稳定性的影响。膜的浓度，以及各种烷烃衍生物的浓度、头基、烷基链长度、不饱和度和双键构型都以系统的方式变化。然后测定每单位膜浓度的两亲物对条带3的变性温度的抑制，以定量每种药物的效力。链长为C8至C24的饱和脂肪酸在C20之前表现出效价单调下降，随后在C22和C24处效价急剧下降。不饱和引起的脂肪酸扰动阴离子交换剂的能力只有轻微的增加，令人惊讶的是，既没有一个趋势的双键的数量，也没有一个显着的cis-transdistinction。花生四烯酸，作为一个例外，是更有效的比任何其他两亲物在不稳定带3。脂肪酸比脂肪胺和脂肪醇的效力高出约三倍;然而，后者进入膜的增强分配在某些膜/缓冲液比率下补偿其降低的内在效力。一个定量模型解释的数据是在一个随附的文件。
The effect of model amphiphiles on the structural stability of the anion exchange protein (band 3) of the human erythrocyte membrane was studied by differential scanning calorimetry. The concentration of membranes, as well as the concentration, head group, alkyl chain length, degree of unsaturation, and double bond configuration of a variety of alkane derivatives were all varied in a systematic way. The depression of the denaturation temperature of band 3 per unit membrane concentration of the amphiphile was then determined in order to quantitate the potency of each drug. Saturated fatty acids of chain length C8to C24displayed a monotonic decrease in potency up to C20, followed by a dramatic diminution in potency at C22and C24. Unsaturation caused only minor increases in the abilities of fatty acids to perturb the anion exchanger, and surprisingly, there was neither a trend for the number of double bonds nor a significantcis-transdistinction. Arachidonic acid, as an exception, was much more effective than any other amphiphile in destabilizing band 3. Fatty acids were about three times more potent than fatty amines and fatty alcohols; however, the enhanced partitioning of the latter into the membrane compensated at certain membrane/buffer ratios for its reduced intrinsic potency. A quantitative model interpretation of the data is presented in an accompanying paper.