Milk fat globule epidermal growth factor 8 (MFG-E8): A novel protein in the mammalian endometrium with putative roles in implantation and placentation
Milk fat globule epidermal growth factor 8 (MFG-E8): A novel protein in the mammalian endometrium with putative roles in implantation and placentation
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DOI：
10.1016/j.placenta.2012.06.015
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发表时间：
2012-10-01
期刊：
影响因子：
3.8
通讯作者：
Oehninger, S.
中科院分区：
文献类型：
--
作者：
Bocca, S. M.;Anderson, S.;Oehninger, S.
目的：MFG-E8是一种新的子宫内膜蛋白，在非子宫组织的组织重塑和血管生成中具有保守的功能。我们的目标是：1。检查MFG-E8蛋白在植入窗口期间的人子宫内膜中、在人子宫内膜细胞系中、在不同胎龄的人胎盘组织中以及在妊娠早期期间的鼠植入部位中的存在;以及2.为研究小鼠着床部位MFG-E8 mRNA表达的调控，采用免疫组化法检测正常人子宫内膜活检组织、人子宫内膜细胞系中MFG-E8蛋白及其受体整合素α v β 3的表达（上皮：石川和HEC-1A，基质：HESC，和内皮：HEEC），在所有妊娠期的人类受孕产物中，以及在交配后第5天和第8天解剖的小鼠着床和着床间部位中。结果：MFG-E8蛋白主要表达于人子宫内膜上皮细胞中，在正常子宫内膜和妊娠产物中的表达均高于正常子宫内膜，而在正常子宫内膜和妊娠产物中MFG-E8 mRNA的表达均低于正常子宫内膜。它也表达在细胞滋养细胞和合体滋养细胞概述绒毛膜绒毛的人胎盘在所有妊娠期，并在小鼠着床部位。MFG-E8 mRNA在小鼠着床部位显著上调，并随着孕龄的增加而增加。结论：MFG-E8在子宫内膜上皮和绒毛膜绒毛中的表达表明其可能在子宫内膜重组过程中的作用，在接受期和哺乳动物正常妊娠相关的事件中。(C)2012爱思唯尔有限公司保留所有权利。
Objectives: MFG-E8 is a novel endometrial protein with conserved functions in tissue remodeling and angiogenesis in non-uterine tissues. Our aims were: 1. To examine the presence of MFG-E8 protein in the human endometrium during the window of implantation, in human endometrial cell lines, in human placental tissue at different gestational ages, and in murine implantation sites during early gestation; and 2. To study the regulation of MFG-E8 mRNA expression in mice implantation sites.Study design: MFG-E8 protein and its receptor integrin alpha v beta 3 were detected by immunostaining in human endometrial biopsies obtained from normal volunteers, in human endometrial cell lines (epithelial: Ishikawa and HEC-1A, stromal: HESC, and endothelial: HEEC), in human products of conception from all trimesters of gestation, and in murine implantation and inter-implantation sites dissected on days 5 and 8 post-coitus. MFG-E8 gene expression was assessed by RT-PCR.Main outcome measures: Immunohistochemical determination of MFG-E8 in endometrium and products of conception as well as relative MFG-E8 mRNA expression in mice implantation sites.Results: MFG-E8 protein was present almost exclusively in the epithelial compartment of human endometrium. It was also expressed in the cytotrophoblasts and syncytiotrophoblasts outlining chorionic villi of the human placenta at all trimesters of gestation, and in murine implantation sites. MFG-E8 mRNA was significantly up-regulated in murine implantation sites and with increased gestational age.Conclusions: MFG-E8 expression in the endometrial epithelium as well as in chorionic villi suggests its possible role in endometrial reorganization during the receptive phase and in events related to normal pregnancy in mammals. (C) 2012 Elsevier Ltd. All rights reserved.