Bioactive glass-ceramic bone repair associated or not with autogenous bone: a study of organic bone matrix organization in a rabbit critical-sized calvarial model
Bioactive glass-ceramic bone repair associated or not with autogenous bone: a study of organic bone matrix organization in a rabbit critical-sized calvarial model
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DOI：
10.1007/s00784-018-2450-x
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发表时间：
2019-01-01
影响因子：
3.4
通讯作者：
Matsumoto, Mariza Akemi
中科院分区：
文献类型：
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作者：
Biguetti, Claudia Cristina;Cavalla, Franco;Matsumoto, Mariza Akemi
本研究的目的是分析骨移植后的骨基质（BMX）组织和使用新型生物活性玻璃陶瓷修复材料和方法30只兔子接受手术双侧顶骨缺损，并根据所用材料分组：（C）对照血凝块，（BG）颗粒状自体骨，（BS）生物活性玻璃陶瓷和BG + BS。术后7，14，和30天，每个标本的片段固定在-80摄氏度的液氮酶谱评价，而其余的固定在10%福尔马林组织学双折射analysis.ResultsThe结果表明，基质组织在实验组相比，C考虑胶原组织显着改善。酶谱分析显示所有组中的pro-MMP-2、pro-MMP-9和活性（a）-MMP-2，表明在此期间总明胶分解活性逐渐降低。在第7天，与其他组相比，BG对pro-MMP-2和9以及a-MMP-2表现出更显著的明胶分解活性。此外，在第7天，53%的激活率（活性形式/[活性形式+潜伏形式]）在C组中明显，在BS组中为33%，而BG组为31%.ConclusionIn general，BS允许产生类似于BG的BMX，具有有组织的胶原沉积和MMP-2和MMP-9的可分解性，临床意义新型骨替代物的评价，具有良好的生物学性能，打开了其作为自体骨移植物的可行且有效的替代物的可能性。
ObjectiveThe aim of the study was to analyze bone matrix (BMX) organization after bone grafting and repair using a new bioactive glass-ceramic (Biosilicate((R))) associated or not with particulate autogenous bone graft.Material and methodsThirty rabbits underwent surgical bilateral parietal defects and divided into groups according to the materials used: (C) controlblood clot, (BG) particulate autogenous bone, (BS) bioactive glass-ceramic, and BG + BS. After 7, 14, and 30days post-surgery, a fragment of each specimen was fixed in -80 degrees C liquid nitrogen for zymographic evaluation, while the remaining was fixed in 10% formalin for histological birefringence analysis.ResultsThe results of this study demonstrated that matrix organization in experimental groups was significantly improved compared to C considering collagenous organization. Zymographic analysis revealed pro-MMP-2, pro-MMP-9, and active (a)-MMP-2 in all groups, showing gradual decrease of total gelatinolytic activity during the periods. At day 7, BG presented more prominent gelatinolytic activity for pro-MMP-2 and 9 and a-MMP-2, when compared to the other groups. In addition, at day 7, a 53% activation ratio (active form/[active form + latent form]) was evident in C group, 33% in BS group, and 31% in BG group.ConclusionIn general, BS allowed the production of a BMX similar to BG, with organized collagen deposition and MMP-2 and MMP-9 disponibility, permitting satisfactory bone remodeling at the late period.Clinical relevanceThe evaluation of new bone substitute, with favorable biological properties, opens the possibility for its use as a viable and efficient alternative to autologous bone graft.