Pharmacological intervention of hippocampal CA3 NMDA receptors impairs acquisition and long-term memory retrieval of spatial pattern completion task
Pharmacological intervention of hippocampal CA3 NMDA receptors impairs acquisition and long-term memory retrieval of spatial pattern completion task
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DOI：
10.1101/lm.1433209
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发表时间：
2009-06-01
影响因子：
2
通讯作者：
Roullet, Pascal
中科院分区：
文献类型：
--
作者：
Fellini, Laetitia;Florian, Cedrick;Roullet, Pascal
模式完成是根据不完整的检索线索检索完整信息的能力。尽管已经证明这种认知能力取决于海马 CA3 区的 NMDA 受体 (NMDA-R)，但这些谷氨酸能受体在模式完成过程中所起的作用尚未明确。在本研究中，我们研究了空间模式完成任务（当从环境中移除一些视觉线索时）中不同记忆阶段（获取、记忆巩固和检索）中 CA3 NMDA-R 的功能，并与标准空间水迷宫任务（当环境中所有视觉线索都可用时）进行比较。因此，我们将集中训练与向 C57BL/6 小鼠背侧海马 CA3 亚区注射 NMDA 受体拮抗剂 (AP5) 相结合。我们的结果表明，NMDA-R 并不涉及标准情况，但在模式完成的获取和长期记忆检索过程中至关重要。这项工作首次证明了 CA3 NMDA-R 在涉及不完整记忆痕迹重新激活的记忆过程中的特定作用，特别是当可用的环境信息量非常有限时。
Pattern completion is the ability to retrieve complete information on the basis of incomplete retrieval cues. Although it has been demonstrated that this cognitive capacity depends on the NMDA receptors (NMDA-Rs) of the hippocampal CA3 region, the role played by these glutamatergic receptors in the pattern completion process has not yet been specified. In the present study, we investigated the function of the CA3 NMDA-Rs during the different memory stages (acquisition, memory consolidation, and retrieval) in a spatial pattern completion task (when some visual cues were removed from the environment) in comparison to a standard spatial water maze task (when all visual cues were available in the environment). Thus, we coupled a massed training with the injection of NMDA-receptor antagonist (AP5) into the CA3 subfield of the dorsal hippocampus of C57BL/6 mice. Our results show that NMDA-Rs are not implicated in a standard situation but are crucial during both acquisition and long-term memory retrieval in pattern completion. This work provides the first evidence of a specific role of CA3 NMDA-Rs during memory process involved in the reactivation of incomplete memory trace, particularly when the amount of environmental information available is strongly limited.