Antibacterial Biomimetic Hybrid Films.
Antibacterial Biomimetic Hybrid Films.
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DOI：
10.1039/c2sm06969e
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发表时间：
2013-02-28
期刊：
影响因子：
3.4
通讯作者：
Composto RJ
中科院分区：
文献类型：
--
作者：
Ferrer MC;Hickok NJ;Eckmann DM;Composto RJ
在这项工作中，我们提出了一种新的方法来制备混合涂层的基础上葡聚糖接枝到基板和嵌入银纳米粒子（银纳米粒子）。首先，在溶液中氧化葡聚糖的存在下原位合成Ag NP。第二，将氧化的葡聚糖暴露于胺官能化的表面，导致葡聚糖的同时接枝和Ag NP在层内的捕获。NP负载由硝酸银的浓度控制，其为2 mM（DEX-Ag 2）和5 mM（DEX-Ag 5）。干膜厚度随着银浓度的增加而增加，从葡聚糖的2nm分别增加到DEX-Ag 2和DEX-Ag 5的7 nm和12 nm。接枝葡聚糖膜显示出直径和高度分别为约50 nm和2 nm的特征。对于DEX-Ag 2和DEX-Ag 5，观察到葡聚糖特征以及单个Ag NP（~ 5 nm）和Ag NP的聚集体。对于DEX-Ag 5，观察到更大和更不规则的聚集体。总的来说，如AFM和UVO研究所示，Ag NP嵌入葡聚糖膜中。就其抗微生物活性而言，DEX-Ag 2比DEX-Ag 5更能抵抗细菌粘附，而DEX-Ag 5又比葡聚糖和硅更好。由于这些抗菌混合涂层可以接枝到各种表面，因此可以设想许多生物医学应用，从涂层植入物到导管。
In this work, we present a novel method to prepare a hybrid coating based on dextran grafted to a substrate and embedded with silver nanoparticles (Ag NPs). First, the Ag NPs are synthesized in situ in the presence of oxidized dextran in solution. Second, the oxidized dextran is exposed to an amine functionalized surface resulting in the simultaneous grafting of dextran and the trapping of Ag NPs within the layer. The NP loading is controlled by the concentration of silver nitrate, which is 2 mM (DEX-Ag2) and 5 mM (DEX-Ag5). The dried film thickness increases with silver nitrate concentration from 2 nm for dextran to 7 nm and 12 nm for DEX-Ag2 and DEX-Ag5, respectively. The grafted dextran film displays features with a diameter and height of ~ 50 nm and 2 nm, respectively. For the DEX-Ag2 and DEX-Ag5, the dextran features as well as individual Ag NPs (~ 5 nm) and aggregates of Ag NPs are observed. Larger and more irregular aggregates are observed for DEX-Ag5. Overall, the Ag NPs are embedded in the dextran film as suggested by AFM and UVO studies. In terms of its antimicrobial activity, DEX-Ag2 resists bacterial adhesion to a greater extent than DEX-Ag5, which in turn is better than dextran and silicon. Because these antibacterial hybrid coatings can be grafted to a variety of surfaces, many biomedical applications can be envisioned, ranging from coating implants to catheters.