Effects of metabolic acidosis and diabetes on the abundance of specific renal mRNAs.
Effects of metabolic acidosis and diabetes on the abundance of specific renal mRNAs.
复制标题
代谢性酸中毒和糖尿病对特定肾 mRNA 丰度的影响。
DOI：
10.1016/0020-711x(89)90187-0
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Watford,M
中科院分区：
文献类型：
--
作者：
Mapes,RE;Watford,M
1.观察了外源性(摄入NH4Cl)和内源性(链脲佐菌素-糖尿病)所致的慢性代谢性酸中毒对大鼠肾脏mRNAs丰度的影响。2.体外翻译总RNA，并对翻译产物进行双向凝胶电泳分析。3.在酸中毒和糖尿病中，翻译产物磷酸烯醇式丙酮酸羧激酶(PEPCK)增加了3.5倍。4.在用NaHCO3治疗的糖尿病大鼠中没有观察到这种增加。5.酸中毒时，另一种翻译产物的丰度增加。6.其他10例糖尿病患者中有10例升高，其中有几例升高，与酸碱状态无关。7.在酸中毒和糖尿病大鼠中，一种翻译产物的丰度降低，而在NaHCO3治疗的糖尿病大鼠中没有，这表明该产物具有酸碱调节作用。8.结果表明，酸中毒反应仅限于少量的肾脏mRNAs，并证实肾脏PEPCK主要受酸碱状态变化的调节。9.它们还表明，糖尿病通过独立于酸碱状态的机制影响特定肾脏mRNAs的丰度。
1. The effects of exogenously (NH4Cl ingestion) and endogenously (streptozotocin-diabetes) generated chronic metabolic acidosis on the abundance of rat renal mRNAs have been examined. 2. Total RNA was translated in vitro and the translation products analyzed by two-dimensional gel electrophoresis. 3. The translation product identified as phosphoenolpyruvate carboxykinase (PEPCK) increased 3.5-fold in both acidosis and diabetes. 4. This increase was not observed in diabetic rats treated with NaHCO3. 5. The abundance of one other translation product increased in acidosis. 6. That of 10 others increased in diabetes, several of which were elevated regardless of acid-base status. 7. The abundance of one translation product decreased in acidosis and diabetes but not in NaHCo3 treated diabetic rats, indicating acid-base regulation of this product. 8. The results establish that the acidosis response is limited to a small number of renal mRNAs and confirm that renal PEPCK is primarily regulated by changes in acid-base status. 9. They also indicate that diabetes affects the abundance of specific renal mRNAs through mechanisms independent of acid-base status.