Enhancing structural integrity of hydrogels by using highly organised melt electrospun fibre constructs
Enhancing structural integrity of hydrogels by using highly organised melt electrospun fibre constructs
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DOI：
10.1016/j.eurpolymj.2015.07.034
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发表时间：
2015-11-01
影响因子：
6
通讯作者：
Hutmacher, Dietmar W.
中科院分区：
文献类型：
--
作者：
Bas, Onur;De-Juan-Pardo, Elena M.;Hutmacher, Dietmar W.
将增材制造技术应用于水凝胶的纤维增强原理，我们将弱水凝胶塑造成机械增强复合材料。我们将明胶-甲基丙烯酰胺（GelMA）和GelMA/透明质酸-甲基丙烯酰胺（HAMA）水凝胶的细胞外基质样结构与通过熔融静电纺丝写入（MEW）制备的高度取向的聚己内酯（PCL）纤维相结合，以实现具有改善的压缩性能的纤维增强GelMA/HAMA复合材料。通过MEW制备具有0度-90度和0度-60度-120度的铺设图案以及400和800 μ m的间距的堆叠纤维。在定制的模具中用水凝胶，即GelMA（10%）和GelMA/HAMA（0.125%、0.25%和0.5%）渗透这些限定的纤维结构，并通过还原-氧化引发体系（过硫酸铵/四甲基乙二胺）交联。在37 ℃下在培养基中进行单轴压缩载荷下，使用集成相机评价了结构的机械性能和变形特征。增强结构显示压缩杨氏模量增加超过35倍。然而，压缩杨氏模量是高度应变率依赖。纤维增强对复合材料结构的泊松比具有特殊影响，从约0.4的值降低到0.01。纤维结构和水凝胶基质之间的高界面表面积被认为是导致结构的机械性能显著增加的主要因素之一。总之，我们已经发现，具有限定的MEW纤维结构的水凝胶的增强在高应变速率下实现了机械性能的显著增加。(C)2015爱思唯尔有限公司版权所有。
Applying additive manufacturing technology to the principles of fibre reinforcement of hydrogels, we have fashioned weak hydrogels into mechanically enhanced composites. We combined the extracellular matrix-like structure of gelatin-methacrylamide (GelMA) and GelMA/hyaluronic acid-methacrylamide (HAMA) hydrogels with highly oriented poly(s-caprolactone) (PCL) fibres fabricated by Melt Electrospinning Writing (MEW) to achieve fibre-reinforced GelMA/HAMA composites with improved compressive properties. Stacked fibres with lay-down patterns of 0 degrees-90 degrees and 0 degrees-60 degrees-120 degrees, and spacing of 400 and 800 mu m were prepared by MEW. These defined fibrous structures were infiltrated with hydrogels, namely GelMA (10%) and GelMA/HAMA (0.125%, 0.25% and 0.5%) in custom-made moulds and crosslinked by a reduction-oxidation initiating system (ammonium persulphate/tetramethylethylenediamine). Mechanical properties and deformation characteristics of the constructs were evaluated under uniaxial compression loading conducted at 37 degrees C in culture media with an integrated camera. Reinforced constructs showed more than a 35-fold-increase of the compressive Young's modulus. However, the compressive Young's moduli were highly strain-rate dependent. The fibre reinforcement has a particular impact on the Poisson's ratio of the composite constructs, decreasing from values of approximately 0.4 to 0.01. The high interfacial surface area between the fibre structure and the hydrogel matrix is believed to be one of the main factors responsible for the significant increase in the mechanical properties of the constructs. In summary, we have found that reinforcement of hydrogels with defined MEW fibre architectures achieves an outstanding increase in the mechanical properties at high strain rates. (C) 2015 Elsevier Ltd. All rights reserved.