Partial Characterization ofaFibroblast-Stimu lating Factor Produced byCloned Murine TLymphocytes
Partial Characterization ofaFibroblast-Stimu lating Factor Produced byCloned Murine TLymphocytes
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克隆鼠T淋巴细胞产生的成纤维细胞刺激因子的部分表征
DOI：
10.1097/00007890-199912150-00027
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发表时间：
1988
期刊：
影响因子：
6.2
通讯作者：
B. Prystowsky
中科院分区：
文献类型：
--
作者：
J. Monroe;A. I. Michael;G. Johnson;S. Phillips;B. Prystowsky
背景：慢性移植物抗宿主病（cGvHD）的标志是皮肤纤维化、挛缩和胶原蛋白含量增加。我们报道了通过局部应用卤常酮（一种胶原蛋白 α1 (I) 基因表达抑制剂）成功治疗一名 cGvHD 患者。方法。每天将含有卤常酮的软膏涂抹在 cGvHD 患者的左侧颈部和肩部。分别通过原位杂交和天狼星红染色评估皮肤活检标本中胶原蛋白α1（I）基因表达和胶原蛋白含量。结果。3个月和6个月后，观察到治疗侧皮肤胶原蛋白合成明显减少，同时颈部旋转增加。停止治疗后，硬化、皮肤紧致度和胶原α1（I）基因表达恢复到基线水平。未观察到不良反应，也未检测到卤常酮的血浆水平。结论。卤常酮可能为cGvHD患者提供一种有前途的新型安全疗法。慢性移植物抗宿主病（cGvHD*）是同种异体骨髓移植（BMT）后患者发生的主要并发症，它类似于结缔组织自身免疫样免疫性疾病，其特征是皮肤的苔藓样或硬皮样病变(1).皮肤纤维化、挛缩和胶原蛋白含量增加是该疾病的标志 (2)。目前的治疗涉及免疫抑制剂，包括甲泼尼龙、环孢素、硫唑嘌呤、沙利度胺、全淋巴结照射，以及最近成功有限的氯法齐明。
Background.In chronic graft-versus-host disease (cGvHD), skin fibrosis, contractures, and an increase in collagen content form the hallmark. We report a successful treatment of a cGvHD patient by topical application of halofuginone, an inhibitor of collagen α1 (I) gene expression.Methods.Halofuginone-containing ointment was applied daily on the left side of the neck and shoulder of a cGvHD patient. Collagen α1 (I) gene expression and collagen content in skin biopsy specimens were evaluated by in situ hybridization and sirius red staining, respectively.Results.After 3 and 6 months, a marked reduction in skin collagen synthesis was observed, accompanied with increase neck rotation on the treated side. After cessation of treatment, the sclerosis, skin tightness, and collagen α1 (I) gene expression returned to baseline level. No adverse effects were observed, and no plasma levels of halofuginone could be detected.Conclusions.Halofuginone may provide a promising novel and safe therapy for cGvHD patients.Chronic graft versus host disease (cGvHD*) is a major complication occurring in patients after allogeneic bone marrow transplants (BMT), it resembles connective tissue-autoimmune-like immunologic disorder, characterized by lichenoid or sclerodermoid lesions of the skin (1). Skin fibrosis, contractures, and an increase in collagen content form the hallmark of the disease (2). Current treatment involves immunosuppressive agents, including methylprednisolone, cyclosporine, and azathioprine, thalidomide, total lymph node irradiation, and recently, with limited success, clofazimine.
血吸虫病中标称抗原特异性 T 细胞克隆反应的同种异体替代。
影响因子：
6.4
作者：
Linette,GP;Lammie,PJ;Phillips,SM
通讯作者：
Phillips,SM
DOI：
--
发表时间：
1986
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
DeClerck,Y;Draper,V;Parkman,R
通讯作者：
Parkman,R
T 淋巴细胞克隆分泌的集落刺激因子的部分纯化和表征。
影响因子：
2.6
作者：
Prystowsky,MB;Ely,JM;Naujokas,MF;Goldwasser,E;Fitch,FW
通讯作者：
Fitch,FW