Spatiotemporal proteomic atlas of multiple brain regions across early fetal to neonatal stages in cynomolgus monkey.
Spatiotemporal proteomic atlas of multiple brain regions across early fetal to neonatal stages in cynomolgus monkey.
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DOI：
10.1038/s41467-023-39411-7
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发表时间：
2023-07-03
影响因子：
16.6
通讯作者：
Si, Wei
中科院分区：
文献类型：
--
作者：
Wei, Jingkuan;Dai, Shaoxing;Yan, Yaping;Li, Shulin;Yang, Pengpeng;Zhu, Ran;Huang, Tianzhuang;Li, Xi;Duan, Yanchao;Wang, Zhengbo;Ji, Weizhi;Si, Wei
胎儿阶段是大脑发育的关键时期。然而，由于采样难度和伦理限制，人脑的蛋白质分子特征和动力学仍不清楚。非人类灵长类动物表现出与人类相似的发育和神经病理学特征。本研究构建了食蟹猴从胎儿早期到新生儿阶段大脑发育的时空蛋白质组图谱。在这里，我们表明（1）不同阶段之间的变异性大于大脑区域之间的变异性，小脑与大脑以及皮质与皮质下区域的比较揭示了胎儿早期到新生儿阶段的区域特异性动态； (2) 与神经疾病相关的蛋白质丰度波动提示胎儿早期出现神经疾病的风险； （3）跨物种分析（人、猴和小鼠）以及蛋白质组和转录组数据之间的比较揭示了蛋白质组特异性和具有mRNA/蛋白质差异的基因。这项研究提供了对灵长类动物胎儿大脑发育的深入了解。为了了解功能基因产物的发育和变化，需要涵盖灵长类动物大脑中胎儿和新生儿大脑发育的蛋白质组学数据。在这里，作者通过构建时空蛋白质组图谱，展示了食蟹猴大脑从胎儿早期到新生儿阶段发育过程中的动态蛋白质组变化。
Fetal stages are critical periods for brain development. However, the protein molecular signature and dynamics of the human brain remain unclear due to sampling difficulty and ethical limitations. Non-human primates present similar developmental and neuropathological features to humans. This study constructed a spatiotemporal proteomic atlas of cynomolgus macaque brain development from early fetal to neonatal stages. Here we showed that (1) the variability across stages was greater than that among brain regions, and comparisons of cerebellum vs. cerebrum and cortical vs. subcortical regions revealed region-specific dynamics across early fetal to neonatal stages; (2) fluctuations in abundance of proteins associated with neural disease suggest the risk of nervous disorder at early fetal stages; (3) cross-species analysis (human, monkey, and mouse) and comparison between proteomic and transcriptomic data reveal the proteomic specificity and genes with mRNA/protein discrepancy. This study provides insight into fetal brain development in primates. Proteomic data covering fetal and neonatal primate brain development in the primate brain is needed to understand development and changes in functional gene products. Here, the authors show the dynamic proteomic changes of the cynomolgus macaque brain during the development from early fetal to neonatal stages by constructing a spatiotemporal proteomic atlas.
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影响因子：
16.2
作者：
Cargnin F;Kwon JS;Katzman S;Chen B;Lee JW;Lee SK
通讯作者：
Lee SK
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56.9
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Sanders, Stephan
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2.9
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影响因子：
64.8
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通讯作者：
Lein ES
影响因子：
13.6
作者：
Li S;Luo H;Lou R;Tian C;Miao C;Xia L;Pan C;Duan X;Dang T;Li H;Fan C;Tang P;Zhang Z;Liu Y;Li Y;Xu F;Zhang Y;Zhong G;Hu J;Shui W
通讯作者：
Shui W