Basal omega-3 fatty acid status affects fatty acid and oxylipin responses to high-dose n3-HUFA in healthy volunteers
Basal omega-3 fatty acid status affects fatty acid and oxylipin responses to high-dose n3-HUFA in healthy volunteers
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DOI：
10.1194/jlr.p025577
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发表时间：
2012-08-01
影响因子：
6.5
通讯作者：
Newman, John W.
中科院分区：
文献类型：
--
作者：
Keenan, Alison H.;Pedersen, Theresa L.;Newman, John W.
受试者的基线FA组成可能影响饮食中高度不饱和omega-3 FAs （n3-HUFA）改变酯化FAs及其氧合代谢产物循环谱的能力。本研究评估了基础n3-HUFA和n3-oxylipin状态对n3-HUFA消耗的反应程度的影响。在治疗前后（4周；11 +/- 2 mg/kg/天n3-HUFA乙酯）采集空腹受试者（n = 30）的血液。用气相色谱-质谱法测定红细胞、血小板和血浆中的酯化FAs。采用LC-MS/MS法测定血浆中酯化氧脂。n3-HUFAs处理增加了所有储存库中的n3-HUFAs，降低了n6-HUFAs，增加了血浆中的n3-羟脂质，但没有显著改变n6-羟脂质浓度。随着受试者基础n3-HUFAs的增加，治疗相关的变化减少，这种行为反映在红细胞膜FAs中20:5n3 + 22:6n3的百分比（即ω -3指数）。为了维持8%的omega-3指数，从而降低心血管疾病的风险，我们的分析建议，对于70公斤的个体，维持剂量为7毫克/公斤/天的n3-HUFA乙酯。（jlr）这些结果表明，基础n3指数可能具有临床应用价值，可用于建立有效的治疗性实验喂养方案，并评估美国农业部膳食指南对n3- hufa摄入量的建议。——基南，a.h., t.l.佩德森，k.i.菲劳斯，m.k.拉尔森，g.c.希勒，j.w.纽曼基础omega-3脂肪酸状态影响健康志愿者对高剂量n3-HUFA的脂肪酸和氧脂反应。[j] .油脂杂志。2012。53: 1662 - 1669。
A subject's baseline FA composition may influence the ability of dietary highly unsaturated omega-3 FAs (n3-HUFA) to change circulating profiles of esterified FAs and their oxygenated metabolites. This study evaluates the influence of basal n3-HUFA and n3-oxylipin status on the magnitude of response to n3-HUFA consumption. Blood was collected from fasting subjects (n = 30) before and after treatment (4 weeks; 11 +/- 2 mg/kg/day n3-HUFA ethyl esters). Esterified FAs were quantified in erythrocytes, platelets, and plasma by GC-MS. Esterified oxylipins were quantified in plasma by LC-MS/MS. Treatment with n3-HUFAs increased n3-HUFAs and decreased n6-HUFAs in all reservoirs and increased plasma n3-oxylipins without significantly changing n6-oxylipin concentrations. As subject basal n3-HUFAs increased, treatment-associated changes decreased, and this behavior was reflected in the percentage of 20:5n3 + 22:6n3 in red blood cell membrane FAs (i.e., the omega-3 index). To maintain an omega-3 index of 8% and thus reduce cardiovascular disease risk, our analyses suggest a maintenance dose of 7 mg/kg/day n3-HUFA ethyl esters for a 70-kg individual.(jlr) These results suggest that the basal n3 index may have clinical utility to establish efficacious therapeutic experimental feeding regimens and to evaluate the USDA Dietary Guidelines recommendations for n3-HUFA consumption.-Keenan, A. H., T. L. Pedersen, K.i Fillaus, M. K. Larson, G. C. Shearer, and J. W. Newman. Basal omega-3 fatty acid status affects fatty acid and oxylipin responses to high-dose n3-HUFA in healthy volunteers. J. Lipid Res. 2012. 53: 1662-1669.