Arabidopsis WRKY71 regulates ethylene-mediated leaf senescence by directly activating EIN2, ORE1 and ACS2 genes
Arabidopsis WRKY71 regulates ethylene-mediated leaf senescence by directly activating EIN2, ORE1 and ACS2 genes
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拟南芥WRKY71通过直接激活EIN2、ORE1和ACS2基因调节乙烯介导的叶片衰老
DOI：
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Fengning Xiang
中科院分区：
文献类型：
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作者：
Yanchong Yu;Yanan Qi;Jinpeng Xu;Xuehuan Dai;Jiacai Chen;Chun-Hai Dong;Fengning Xiang
Leaf senescence is a pivotal step in the last stage of the plant life cycle and is influenced by various external.and endogenous cues. A series of reports have indicated the involvement of the WRKY transcription factors.in regulating leaf senescence, but the molecular mechanisms and signaling pathways remain largely.unclear. Here we provide evidence demonstrating that WRKY71 acts as a positive regulator of leaf senes-.cence in Arabidopsis. WRKY71-1D, an overexpressor of WRKY71, exhibited early leaf senescence, while.wrky71-1, the WRKY71 loss-of-function mutant, displayed delayed leaf senescence. Accordingly, a set of.senescence-associated genes (SAGs) were substantially elevated in WRKY71-1D but markedly decreased in.wrky71-1. Chromatin immunoprecipitation assays indicated that WRKY71 can bind directly to the promoters.of SAG13 and SAG201. Transcriptome analysis suggested that WRKY71 might mediate multiple cues to.accelerate leaf senescence, such as abiotic stresses, dark and ethylene. WRKY71 was ethylene inducible,.and treatment with the ethylene precursor 1-amino-cyclopropane-1-carboxylic acid enhanced leaf senes-.cence in WRKY71-1D but caused only a marginal delay in leaf senescence in wrky71-1. In vitro and in vivo.assays demonstrated that WRKY71 can directly regulate ETHYLENE INSENSITIVE2 (EIN2) and ORESARA1.(ORE1), genes of the ethylene signaling pathway. Consistently, leaf senescence of WRKY71-1D was obvi-.ously retarded in the ein2-5 and nac2-1 mutants. Moreover, WRKY71 was also proved to interact with ACS2.in vitro and in vivo. Treatment with AgNO 3 and aminoethoxyvinylglycine and acs2-1 could greatly arrest the.leaf senescence of WRKY71-1D. In conclusion, our data revealed that WRKY71 mediates ethylene signaling.and synthesis to hasten leaf senescence in Arabidopsis.
