Hedgehog signaling regulates size of the dorsal aortae and density of the plexus during avian vascular development.
Hedgehog signaling regulates size of the dorsal aortae and density of the plexus during avian vascular development.
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DOI：
10.1002/dvdy.22600
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发表时间：
2011-06
期刊：
影响因子：
--
通讯作者：
Krieg PA
中科院分区：
文献类型：
--
作者：
Moran CM;Salanga MC;Krieg PA
hedgehog （Hh）家族分泌的生长因子对胚胎血管的发育至关重要。缺乏Hh功能的胚胎有丰富的内皮细胞，但不能形成血管索或管状管。然而，Hh信号在血管模式和形态发生的后期方面的作用在很大程度上未被探索。我们已经使用小分子抑制剂和激动剂来改变鸡胚胎中Hh信号通路的活性。当脐带形成后加入环巴胺时，主动脉细胞形成管，但这些管小且无组织，相邻血管丛的密度降低。Hh通路与SAG的激活导致主动脉扩大和神经丛密度增加。内皮细胞丝状伪足的数量与Hh信号水平相关。这些研究表明，要实现正常的血管模式，必须严格调节Hh信号水平。
Signaling by the hedgehog (Hh) family of secreted growth factors is essential for development of embryonic blood vessels. Embryos lacking Hh function have abundant endothelial cells but fail to assemble vascular cords or lumenized endothelial tubes. However, the role of Hh signaling during later aspects of vascular patterning and morphogenesis is largely unexplored. We have used small molecule inhibitors and agonists to alter activity of the Hh signaling pathway in the chick embryo. When cyclopamine is added after cord formation, aortal cells form tubes, but these are small and disorganized and the density of the adjacent vascular plexus is reduced. Activation of the Hh pathway with SAG leads to formation of enlarged aortae and increased density of the plexus. The number of endothelial cell filopodia is found to correlate with Hh signaling levels. These studies show that Hh signaling levels must be tightly regulated for normal vascular patterning to be achieved.