Optimization of Ultrasonic-Assisted Extraction of Total Saponins from Eclipta prostrasta L. Using Response Surface Methodology
Optimization of Ultrasonic-Assisted Extraction of Total Saponins from Eclipta prostrasta L. Using Response Surface Methodology
复制标题
DOI：
10.1111/j.1750-3841.2012.02869.x
复制
发表时间：
2012-09-01
影响因子：
3.9
通讯作者：
Jiang, Jian-Guo
中科院分区：
文献类型：
--
作者：
Hu, Ting;Guo, Yan-Yun;Jiang, Jian-Guo
黄花是一种富含皂苷的中草药，具有很强的抗病毒和抗肿瘤活性。研究了超声辅助提取法快速提取牛蒡中皂苷的方法。采用紫外/可见分光光度法测定了苦参总皂苷的含量。采用单因素实验设计和Box-Behnken实验设计，考察乙醇浓度、提取时间、温度、液料比等因素对提取效果的影响。采用响应面法（RSM）对4个自变量、每个变量在3个水平上的提取条件进行优化，以获得前列藤总皂苷的最高得率。结果表明：乙醇浓度为70%，提取时间为3 h，提取温度为70℃，液料比为14:1。相应皂苷含量为2.096%。所建立的数学模型与实验数据吻合较好。实际应用：虽然由于其多种生物活性，作为功能性食品或传统药物有广泛的应用，但这些特性受到其粗提取物的限制。总皂苷是前列藤的主要活性成分。本研究优化了黄芪总皂苷的提取工艺，为进一步研究提供了有益的参考信息，并为相关行业的实践提供了有益的指导。
Eclipta prostrasta L. is a traditional Chinese medicine herb, which is rich in saponins and has strong antiviral and antitumor activities. An ultrasonic-assisted extraction (UAE) technique was developed for the fast extraction of saponins from E. prostrasta. The content of total saponins in E. prostrasta was determined using UV/vis spectrophotometric methods. Several influential parameters like ethanol concentration, extraction time, temperature, and liquid/solid ratio were investigated for the optimization of the extraction using single factor and Box-Behnken experimental designs. Extraction conditions were optimized for maximum yield of total saponins in E. prostrasta using response surface methodology (RSM) with 4 independent variables at 3 levels of each variable. Results showed that the optimization conditions for saponins extraction were: ethanol concentration 70%, extraction time 3 h, temperature 70 degrees C, and liquid/solid ratio 14:1. Corresponding saponins content was 2.096%. The mathematical model developed was found to fit well with the experimental data. Practical Application: Although there are wider applications of Eclipta prostrasta L. as a functional food or traditional medicine due to its various bioactivities, these properties are limited by its crude extracts. Total saponins are the main active ingredient of E. prostrasta. This research has optimized the extraction conditions of total saponins from E. prostrasta, which will provide useful reference information for further studies, and offer related industries with helpful guidance in practice.