Basic studies on sonoporation with size- and position-controlled microbubbles adjacent to cells.
Basic studies on sonoporation with size- and position-controlled microbubbles adjacent to cells.
复制标题
利用邻近细胞的尺寸和位置控制微泡进行声孔作用的基础研究。
DOI：
10.1109/ultsym.2013.0014
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Uchida K.
中科院分区：
文献类型：
--
作者：
Kudo N;Tanaka Y;Uchida K.
我们一直在研究一种声波修复技术，该技术使用短脉冲超声波一次性暴露于附着有微泡的细胞。在这里，我们介绍了一种新的观察系统，用于阐明声调作用的机制。倒置式显微镜配备了光学镊子，可以控制微气泡的位置。拉盖尔-高斯(LG)光束是一种中心有暗斑的高斯光束，用来捕获折射率低于周围水的微泡。LG光束是使用纯相位空间光调制器和1065 nm激光产生的。基本实验结果表明，该镊子可捕获悬浮在水中的直径为1~30μm的气泡，捕获直径为5μm的气泡的最小光功率约为10 mW。利用放置在细胞膜上或放置在与细胞分离的位置上的大小和位置控制的气泡进行了声孔作用实验，结果表明了新开发的系统对于阐明声孔作用机理的重要性。
We have been studying a sonoporation technique that uses one-shot exposure of short-pulsed ultrasound to cells with attached microbubbles. Here, we introduce a new observation system developed for elucidation of sonoporation mechanisms. An inverted-type microscope was equipped with optical tweezers that enable position control of a microbubble. A Laguerre-Gaussian (LG) beam, a Gaussian beam with a dark spot at the center, was used to trap a microbubble with a refractive index lower than that of the surrounding water. The LG beam was produced using a phase-only spatial light modulator and 1,065-nm laser light. Results of basic experiments showed that the tweezers can trap bubbles of 1-30 μm in diameter suspended in water, and the minimum optical power for trapping bubbles of 5 μm in diameter was around 10 mW. Sonoporation experiments were carried out using size- and position-controlled bubbles placed on cell membranes or placed at separated positions from cells, and the results indicated the importance of the newly developed system for elucidation of sonoporation mechanisms.