Near-infrared emitting probes for biological imaging: Organic fluorophores, quantum dots, fluorescent proteins, lanthanide(III) complexes and nanomaterials
Near-infrared emitting probes for biological imaging: Organic fluorophores, quantum dots, fluorescent proteins, lanthanide(III) complexes and nanomaterials
复制标题
DOI：
10.1016/j.jlumin.2016.09.058
复制
发表时间：
2017-09-01
影响因子：
3.6
通讯作者：
Petoud, Stephane
中科院分区：
文献类型：
--
作者：
Martinic, Ivana;Eliseeva, Svetlana V.;Petoud, Stephane
荧光光学成像是一种高灵敏度和功能强大的工具，在临床前和临床研究中具有多种体外和体内应用。本文综述了近红外（NIR）区域的光学成像，因为由于在这些波长处最小的自发荧光和减少的光散射，可以实现改善的信噪比和光通过组织的更深的穿透。特别地，讨论了在生物诊断窗口（650-1450 nm）中吸收和发射的成像剂。介绍了四种不同类型的探针的物理性质和化学结构或组成的特殊性：（i）有机荧光团，（ii）荧光蛋白（FP），（iii）半导体纳米晶体（量子点）和（iv）镧系元素（III）基复合物和纳米材料。这些探针的优点和缺点，商业可用性和毒性以及选择的应用进行了讨论。一个特别注意的是给予镧系元素（III）的化合物，由于其独特的光学性能，例如尖锐的发射带与微环境的敏感性最小，激发和发射波长之间的巨大差异和对光漂白的强烈抵抗。这种探针的使用带来了额外的视角，并促进了光学成像新策略的发展，包括实时实验和新的诊断方法。(C)2017爱思唯尔B. V.保留所有权利。
Fluorescence optical imaging is a highly sensitive and powerful tool with diverse in vitro and in vivo applications for preclinical and clinical studies. This review is focused on the optical imaging in the near-infrared (NIR) region since improved signal-to-noise ratio and deeper penetration of light through tissues could be achieved due to the minimal autofluorescence and reduced light scattering at these wavelengths. In particular, imaging agents absorbing and emitting in the biological diagnostic window (650-1450 nm) are discussed. The photophysical properties and particularities of chemical structures or compositions of four different families of probes: (i) organic fluorophores, (ii) fluorescent proteins (FPs), (iii) semi-conductor nanocrystals (quantum dots) and (iv) lanthanide(III)-based complexes and nanomaterials are presented. Advantages and drawbacks, commercial availability and toxicity as well as selected applications of these probes are discussed. A specific attention is given to lanthanide(III)-based compounds due to their unique optical properties, e.g. sharp emission bands with minimal sensitivity to the microenvironment, large differences between excitation and emission wavelengths and strong resistance toward photobleaching. The use of such probes brings additional perspectives and facilitates developments of novel strategies in optical imaging including real-time experiments and new approaches for diagnostic. (C) 2017 Elsevier B.V. All rights reserved.