Isolation of mesenchymal stem cells from the mandibular marrow aspirates
Isolation of mesenchymal stem cells from the mandibular marrow aspirates
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DOI：
10.1016/j.tripleo.2011.05.032
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发表时间：
2011-12-01
期刊：
影响因子：
--
通讯作者：
Oh, Se-Hoon
中科院分区：
文献类型：
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作者：
Lee, Bu-Kyu;Choi, Su-Jin;Oh, Se-Hoon
Objective.本研究的目的是确定间充质干细胞（MSCs）是否可以从下颌骨骨髓穿刺液中获得。在5例患者中，从下颌骨和髂嵴获得10 mL骨髓穿刺液。第2代细胞经荧光激活细胞分选仪（FACS）鉴定，第5代细胞体外分化为3种间充质组织。从下颌骨抽取的每10 mL骨髓中，MSC的平均总细胞产量为2.8 +/- 1.8 x 10（5）。从下颌骨和髂嵴分离的MSC的免疫表型高度相似，如通过FACS分析所示。从所有吸出物中分离的所有MSC均成功分化为中胚层细胞类型，如骨细胞、软骨细胞和脂肪细胞。可以从下颌骨吸出物中分离出MSC，为将来的牙科和颅颌面疾病的治疗提供替代的可获得的MSC来源。(Oral外科口腔医学口腔病理学口腔放射学Endod 2011;112：e86-e93）
Objective. The aim of this study was to determine if mesenchymal stem cells (MSCs) could be obtained from mandible marrow aspirates.Study design. In 5 patients, 10 mL marrow aspirates were obtained from both the mandible and the iliac crest. Second passage MSCs were characterized by fluorescence-activated cell sorting (FACS) analysis using MSC-specific cell surface marker, and fifth-passage cells were differentiated into 3 mesenchymal tissues in vitro.Results. Average total cell yields of MSCs were 2.8 +/- 1.8 x 10(5) per 10 mL marrow aspirates from the mandible. Immunophenotypes of MSCs isolated from the mandible and iliac crest were highly similar, as indicated by FACS analysis. Differentiation into mesodermal cell types, such as osteocytes, chondrocytes, and adipocytes, was successfully achieved in all MSC isolates from all aspirates.Conclusions. The MSCs can be isolated from the mandibular aspirates, providing an alternative accessible source of MSCs for the treatment of future dental and craniomaxillofacial diseases. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011;112:e86-e93)