Characterization of ribosomal binding and antibacterial activities using two orthogonal high-throughput screens.
Characterization of ribosomal binding and antibacterial activities using two orthogonal high-throughput screens.
复制标题
使用两个正交高通量筛选表征核糖体结合和抗菌活性。
DOI：
10.1128/aac.00671-13
复制
发表时间：
2013
影响因子：
4.9
通讯作者：
Arya,DevP
中科院分区：
文献类型：
--
作者：
King,Ada;Watkins,Derrick;Kumar,Sunil;Ranjan,Nihar;Gong,Changjun;Whitlock,Jarred;Arya,DevP
We report here the affinity and antibacterial activity of a structurally similar class of neomycin dimers. The affinity of the dimer library for rRNA was established by using a screen that measures the displacement of fluorescein-neomycin (F-neo) probe from RNA. A rapid growth inhibition assay using a single drug concentration was used to examine the antibacterial activity. The structure-activity relationship data were then rapidly analyzed using a two-dimensional ribosomal binding-bacterial inhibition plot analysis.
