Spermine and spermidine mediate protection against oxidative damage caused by hydrogen peroxide
Spermine and spermidine mediate protection against oxidative damage caused by hydrogen peroxide
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DOI：
10.1007/s00726-007-0513-4
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发表时间：
2007-08-01
期刊：
影响因子：
3.5
通讯作者：
Casero, R. A., Jr.
中科院分区：
文献类型：
--
作者：
Rider, J. E.;Hacker, A.;Casero, R. A., Jr.
多胺亚精胺和精胺被认为在保护DNA免受活性氧侵害方面具有不同的功能。在氧化应激反应中，将不能合成精胺的小鼠成纤维细胞与正常成纤维细胞在天然状态和多胺耗尽状态下的生长和存活进行了比较。这些研究的结果表明，当存在于正常或超生理浓度时，亚精胺或精胺都可以保护细胞免受活性氧的侵害。然而，当多胺池被药理学操纵以产生低水平的精胺或亚精胺为主的细胞时，精胺似乎更有效。重要的是，当细胞同时缺乏谷胱甘肽和内源性多胺时，与单独缺乏谷胱甘肽相比，它们对过氧化氢的敏感性增加，这表明多胺不仅在保护细胞免受氧化应激方面发挥作用，而且这种作用与谷胱甘肽所起的作用不同。
The polyamines spermidine and spermine have been hypothesized to possess different functions in the protection of DNA from reactive oxygen species. The growth and survival of mouse fibroblasts unable to synthesize spermine were compared to their normal counterparts in their native and polyamine-depleted states in response to oxidative stress. The results of these studies suggest that when present at normal or supraphysiological concentrations, either spermidine or spermine can protect cells from reactive oxygen species. However, when polyamine pools are pharmacologically manipulated to produce cells with low levels of predominately spermine or spermidine, spermine appears to be more effective. Importantly, when cells are depleted of both glutathione and endogenous polyamines, they exhibit increased sensitivity to hydrogen peroxide as compared to glutathione depletion alone, suggesting that polyamines not only play a role in protecting cells from oxidative stress but this role is distinct from that played by glutathione.