Long-term renewal of hair follicles from clonogenic multipotent stem cells
Long-term renewal of hair follicles from clonogenic multipotent stem cells
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DOI：
10.1073/pnas.0507250102
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发表时间：
2005-10-11
影响因子：
11.1
通讯作者：
Barrandon, Y
中科院分区：
文献类型：
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作者：
Claudinot, S;Nicolas, M;Barrandon, Y
成体干细胞对于组织更新、再生和修复是必不可少的，它们在培养中的扩增对再生医学至关重要。以大鼠的胡须毛囊为模型系统，我们证明：(1)克隆形成是毛囊成体干细胞的固有属性；(2)经140倍体培养后，这些干细胞移植到新生小鼠皮肤的真皮-表皮交界处，形成部分或全部发育中的毛囊；(4)进入毛囊的干细胞是多能的，因为它们产生了毛囊和皮脂腺的所有谱系；(4)单个培养的干细胞的后代可以产生数千个毛囊；(V)培养的干细胞表达自我更新基因Bmi1和Zfp145；(Vi)多个干细胞参与单个毛球的形成；以及(Vii)胡须毛囊中的干细胞比通过标记保留实验所预期的要多得多。
Adult stem cells are essential for tissue renewal, regeneration, and repair, and their expansion in culture is of paramount importance for regenerative medicine. Using the whisker follicle of the rat as a model system, we demonstrate that (i) clonogenicity is an intrinsic property of the adult stem cells of the hair follicle; (h) after cultivation for >140 doublings, these stem cells, transplanted to the dermo-epidermal junction of newborn mouse skin, form part or all of the developing follicles; (fit) the stem cells incorporated into follicles are multipotent, because they generate all of the lineages of the hair follicle and sebaceous gland; (iv) thousands of hair follicles can be generated from the progeny of a single cultivated stem cell; (v) cultured stem cells express the self-renewal genes Bmi1 and Zfp145; (vi) several stem cells participate in the formation of a single hair bulb; and (vii) there are many more stem cells in whisker follicles than could be anticipated from label-retaining experiments.