RAPID METHOD FOR QUANTITATIVE DETERMINATION OF MICROTUBULE PROTEIN USING DEAE-CELLULOSE FILTERS
RAPID METHOD FOR QUANTITATIVE DETERMINATION OF MICROTUBULE PROTEIN USING DEAE-CELLULOSE FILTERS
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DOI：
10.1016/0003-2697(72)90046-2
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发表时间：
1972-01-01
影响因子：
2.9
通讯作者：
BORISY, GG
中科院分区：
文献类型：
--
作者：
BORISY, GG
本文建立了一种用~ 3 H-秋水仙素和DEAE-纤维素滤纸快速定量测定细胞提取液中微管蛋白含量的方法。该方法将检测不到0.1 μg微管蛋白，并且在高达300 μg蛋白质时呈线性。
A rapid quantitative method has been developed for determining specifically the amount of microtubule protein in cell extracts using a DEAE-cellulose filter paper assay and3H-colchicine. The method will detect less than 0.1 μg of microtubule protein and is linear up to 300 μg protein.