Sox7 is dispensable for primitive endoderm differentiation from mouse ES cells.
Sox7 is dispensable for primitive endoderm differentiation from mouse ES cells.
复制标题
Sox7对于与小鼠ES细胞的原始内胚层分化是可分配的。
DOI：
10.1186/s12861-015-0079-4
复制
发表时间：
2015-10-16
影响因子：
--
通讯作者：
Niwa H
中科院分区：
文献类型：
--
作者：
Kinoshita M;Shimosato D;Yamane M;Niwa H
原始内胚层是胚泡中与上胚层分离的细胞谱系，并产生壁层和内脏内胚层。Sox 7是SoxF基因家族的成员，其特异性地表达于晚期胚泡的原始内胚层中，尽管其在该细胞谱系中的功能尚不清楚。在这里，我们的特点Sox 7在原始内胚层分化小鼠胚胎干细胞（ES）作为一个模型系统的功能。我们发现，异位表达的Sox 7在ES细胞有触发分化成原始内胚层样细胞的边际效应。我们还表明，在ES细胞中Sox 7的靶向破坏不影响在胚状体形成中分化成原始内胚层细胞，以及通过Gata 6的强制表达。这些数据表明，Sox 7的功能是补充性的，而不是从ES细胞分化所必需的。本文的在线版本（doi：10.1186/s12861-015-0079-4）包含补充材料，可供授权用户使用。
Primitive endoderm is a cell lineage segregated from the epiblast in the blastocyst and gives rise to parietal and visceral endoderm. Sox7 is a member of the SoxF gene family that is specifically expressed in primitive endoderm in the late blastocyst, although its function in this cell lineage remains unclear. Here we characterize the function of Sox7 in primitive endoderm differentiation using mouse embryonic stem (ES) cells as a model system. We show that ectopic expression of Sox7 in ES cells has a marginal effect on triggering differentiation into primitive endoderm-like cells. We also show that targeted disruption of Sox7 in ES cells does not affect differentiation into primitive endoderm cells in embryoid body formation as well as by forced expression of Gata6. These data indicate that Sox7 function is supplementary and not essential for this differentiation from ES cells. The online version of this article (doi:10.1186/s12861-015-0079-4) contains supplementary material, which is available to authorized users.