DIGESTION OF HIGHLY MODIFIED BACTERIOPHAGE DNA BY RESTRICTION ENDONUCLEASES
DIGESTION OF HIGHLY MODIFIED BACTERIOPHAGE DNA BY RESTRICTION ENDONUCLEASES
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DOI：
10.1093/nar/10.5.1579
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发表时间：
1982-01-01
影响因子：
14.9
通讯作者：
EHRLICH, M
中科院分区：
文献类型：
--
作者：
HUANG, LH;FARNET, CM;EHRLICH, M
已经检查了三十种II型限制性内切核酸酶切割五种不同类型的高度修饰的DNA的能力。DNA底物来源于相对较大的噬菌体基因组，其含有全部或大部分在5位取代的胞嘧啶或胸腺嘧啶残基。这些取代基是质子（PBS 1 DNA）、羟甲基（SP 01 DNA）、甲基（XP 12 DNA）、葡糖基化羟甲基（T4 DNA）或磷酸葡糖醛酸化的葡糖基化4，5-二羟基戊基（SP15 DNA）。虽然PBS 1 DNA和SP 01 DNA被大多数酶消化，但它们被其中许多酶切割的速度比正常DNA慢得多。富含5-甲基胞苷的XP 12 DNA和多重修饰的T4和SP15 DNA对大多数内切酶具有抗性。唯一能切割这五种DNA的酶是TaqI，它能将它们广泛地片段化。
The ability of thirty Type II restriction endonucleases to cleave five different types of highly modified DNA has been examined. The DNA substrates were derived from relatively large bacteriophage genomes which contain all or most of the cytosine or thymine residues substituted at the 5-position. These substituents were a proton (PBS1 DNA), a hydroxymethyl group (SP01 DNA), a methyl group (XP12 DNA), a glucosylated hydroxymethyl group (T4 DNA), or a phosphoglucuronated, glucosylated 4,5-dihydroxypentyl group (SP15 DNA). Although PBS1 DNA and SP01 DNA were digested by most of the enzymes, they were cleaved much more slowly than was normal DNA by many of them. 5-Methyl-cytoslne-rich XP12 DNA and the multiply modified T4 and SP15 DNAs were resistant to most of these endonucleases. The only enzyme that cleaved all five of these DNAs wasTaqI, which fragmented them extensively.