SIMULTANEOUS ANALYSIS OF SAPONINS IN GINSENG-RADIX BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
SIMULTANEOUS ANALYSIS OF SAPONINS IN GINSENG-RADIX BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
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DOI：
10.1248/cpb.43.137
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发表时间：
1995-01-01
影响因子：
1.7
通讯作者：
KUBO, M
中科院分区：
文献类型：
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作者：
SAMUKAWA, K;YAMASHITA, H;KUBO, M
人参酸根中含有约25个人参皂苷，它们的结构已被阐明。虽然一些重要的中性和酸性皂苷已被检测到，但其他皂苷还没有被检测到。采用改进的C-18色谱柱和CH3CN-H2O梯度系统，不仅可以测定红参中具有代表性的人参皂苷[20(R)-人参皂苷-Rg(2)和-Rh-1，20(S，R)-人参皂苷-Rg(3)和-Rs(1)]中的人参皂苷，还可以测定少量的人参皂苷[人参皂苷-Rb-3，-Rg(2)，-Rh-1和Ququienoside-R1]。此外，将该方法应用于人参提取物的洗脱液中，实现了22种中性和酸性人参皂苷的同时分离。利用该方法对人参皂苷进行定性和定量分析，为人参及其商品的质量评价提供了一种有效手段。
The presence of about 25 ginsenosides in Ginseng Radix acid their structures have already been elucidated. Although some important neutral- and acidic-saponins have been detected by high performance liquid chromatography (HPLC), other saponins have not yet been examined by HPLC. Our present improved method for the detection of ginsenosides by HPLC with a C-18 column and a CH3CN-H2O gradient system enabled the measurement of not only the representative ginsenosides in Red Ginseng [20(R)-ginsenoside-Rg(2) and -Rh-1, 20(S,R)-ginsenosides-Rg(3) and -Rs(1)] but also the minor ginsenosides [ginsenosides-Rb-3, -Rg(2), -Rh-1 and quinquenoside-R1]. Furthermore, the application of this method after a little modification of the eluent in particular to an extract of Ginseng Radix achieved simultaneous separation of 22 neutral- and acidic-ginsenosides. The qualitative and quantitative analysis of ginsenosides using the present method offers an efficient means of evaluating Ginseng Radix and commercial products containing it.