Systemic antiinflammatory corticosteroid reduces mechanical pain behavior, sympathetic sprouting and elevation of proinflammatory cytokines in a rat model of neuropathic pain
Systemic antiinflammatory corticosteroid reduces mechanical pain behavior, sympathetic sprouting and elevation of proinflammatory cytokines in a rat model of neuropathic pain
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DOI：
10.1097/01.anes.0000278907.37774.8d
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发表时间：
2007-09-01
期刊：
影响因子：
8.8
通讯作者：
Zhang, Jun-Ming
中科院分区：
文献类型：
--
作者：
Li, Huiqing;Xie, Wenrui;Zhang, Jun-Ming
背景资料：慢性疼痛模型通常被定义为神经损伤或炎症模型，但最近的工作表明炎症过程在神经损伤引起的疼痛中很重要。在大鼠脊神经结扎模型中，作者研究了全身性皮质类固醇曲安奈德（TA）对轴突切断的感觉神经节中细胞因子蛋白谱和交感神经发芽，感觉神经元兴奋性，结果：术后第3天，观察到单核细胞趋化蛋白-1、生长相关癌基因（GRO/KC或CXCL 1）和白细胞介素（IL）-6显著增加（5- 16倍），而IL-4和IL-2水平下降超过4倍。细胞因子增加;并且在损伤时开始给予TA使感觉神经节中的交感神经篮形成的数量向正常值减少。TA不能恢复IL-4和IL-2水平。全身性TA也降低了放电率和突发活动的发生率，但不是自发活动的总体发生率，在大型和中型神经元。术后第3天，TA可减轻机械性超敏反应，停药后4天仍有一定效果。然而，开始TA在第7天是无效的。结论：脊神经损伤模型的几个组成部分是响应皮质类固醇，这表明炎症过程是重要的神经病理性疼痛的发展。在损伤时开始给予TA有效的观察结果表明，类固醇治疗可能会改变涉及神经损伤的外科手术（如截肢或疝修补术）后慢性疼痛的发展。
Background: Chronic pain models are commonly defined as either nerve-injury or inflammation models, but recent work suggests inflammatory processes are important in nerve injury-induced pain.Methods: In the rat spinal nerve ligation model, the authors examined effects of systemic corticosteroid triamcinolone acetonide (TA) on the cytokine protein profile and sympathetic sprouting in the axotomized sensory ganglia, excitability of sensory neurons, and mechanical sensitivity.Results: By postoperative day 3, marked increases (5- to 16-fold) in monocyte chemoattractant protein-1, growth-related oncogene (GRO/KC or CXCL1), and interleukin (IL)-6 were observed, whereas IL-4 and IL-2 levels fell more than fourfold. The increased cytokines; and number of sympathetic basket formations in the sensory ganglia were reduced toward normal values by TA given starting at the time of injury. Interleukin-4 and IL-2 levels were not restored by TA. Systemic TA also reduced the firing rate and incidence of bursting activity, but not the overall incidence of spontaneous activity, in large- and medium-sized neurons. Mechanical hypersensitivity on postoperative day 3 was reduced by TA, and some effect could still be observed 4 days after cessation of TA. However, starting TA at day 7 was ineffective.Conclusions: Several components of the spinal nerve injury model are responsive to corticosteroid, suggesting inflammatory processes are important in the development of neuropathic pain. The observation that TA was effective when given starting at the time of injury suggests that steroid treatment might alter the development of chronic pain after surgical procedures that involve nerve injury, such as amputation or hernia repair.