Effect of growth conditions on advantages of hup- strain for H2 photoproduction by Rubrivivax gelatinosus
Effect of growth conditions on advantages of hup- strain for H2 photoproduction by Rubrivivax gelatinosus
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生长条件对 hup 菌株光产 H2 优势的影响
DOI：
10.1016/j.ijhydene.2016.12.074
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
and A. Tsygankov
中科院分区：
文献类型：
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作者：
T. Laurinavichene;M. Kitashima;K. V. P. Nagashima;T. Sato;H. Sakurai;K. Inoue;and A. Tsygankov
比较了 hup 突变体和明胶红枣亲本菌株 RL2 的生长培养物的 H2 光生产。我们检查了不同底物、空气和氮气的存在、培养物摇动、接种浓度的影响。在低接种浓度下，hup-菌株在微氧条件下表现出比亲本菌株显着的优势，而在N2-Ar气氛下，其较低并在厌氧条件（仅Ar）下消失。当使用低还原度底物（苹果酸和琥珀酸）时，这一优势非常明显。在微需氧条件下和存在 N2 的情况下摇动培养物完全阻止了两种菌株的 H2 产生。与厌氧条件不同，高接种浓度在微需氧条件下和存在 N2 的情况下抑制 H2 产生。随着接种浓度的增加，氢气产量不是逐渐而是逐步减少，这意味着一些代谢变化。 hup-菌株产生的氢气似乎比亲本菌株更能耐受空气痕迹。
H2photoproduction by growing cultures ofhup−mutant and parental strain RL2 ofRubrivivax gelatinosuswas compared. We checked the influence of different substrates, presence of air and N2, culture shaking, inoculum concentrations. At low inoculum concentration,hup−strain demonstrated significant advantage over the parental strain in microaerobic conditions, while under N2–Ar atmosphere it was lower and vanished in anaerobic conditions (Ar only). This advantage was evident when using substrates with low degree of reduction (malate and succinate). Culture shaking under microaerobic conditions and in presence of N2completely prevented H2production by both strains. The high inoculum concentration inhibited H2production under microaerobic conditions and in presence of N2, unlike to anaerobic conditions. With inoculum concentration increase, H2production decreased not gradually but stepwise which means some metabolic shift. H2production byhup−strain seems to be more tolerant to air traces than by parental strain.