Gene mapping with recombinant inbreds in maize.
Gene mapping with recombinant inbreds in maize.
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DOI：
10.1093/genetics/118.3.519
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发表时间：
1988-03
期刊：
影响因子：
3.3
通讯作者：
Benjamin Burr;F. Burr;Keith H. Thompson;C. AlbertsonJT;Charles;-W.;Stuber
中科院分区：
文献类型：
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作者：
Benjamin Burr;F. Burr;Keith H. Thompson;C. AlbertsonJT;Charles;-W.;Stuber
重组玉米自交系已被开发用于染色体定位的分子探针快速定位。利用T232 X CM37和CO159 X Tx303的F2个群体构建了两个重组近交系。一个主要基于同工酶和限制性片段长度多态性的遗传图谱已经产生，几乎覆盖了整个玉米基因组。为了绘制一个新基因的图谱，研究者只需确定其等位基因在重组自交系中的分布，然后用计算机将其与所有先前绘制的基因座的分布进行比较。重组自交系和相关数据库的可用性为玉米新分子标记的定位提供了有效手段。
Recombinant inbred lines of maize have been developed for the rapid mapping of molecular probes to chromosomal location. Two recombinant inbred families have been constructed from F2 populations of T232 X CM37 and CO159 X Tx303. A genetic map based largely on isozymes and restriction fragment length polymorphisms has been produced that covers virtually the entire maize genome. In order to map a new gene, an investigator has only to determine its allelic distribution among the recombinant inbred lines and then compare it by computer with the distributions of all previously mapped loci. The availability of the recombinant inbreds and the associated data base constitute an efficient means of mapping new molecular markers in maize.