Gibberellins in dark- and red-light-grown shoots of dwarf and tall cultivars of Pisum sativum: The quantification, metabolism and biological activity of gibberellins in Progress no. 9 and Alaska
Gibberellins in dark- and red-light-grown shoots of dwarf and tall cultivars of Pisum sativum: The quantification, metabolism and biological activity of gibberellins in Progress no. 9 and Alaska
复制标题
豌豆矮秆和高秆品种的暗光和红光生长芽中的赤霉素：进展号中赤霉素的定量、代谢和生物活性。
作者：
V. Sponsel
对阿拉斯加（Le Le，高）和进步 9 号（le le，矮）这两个豌豆品种在黑暗或连续红光下的茎生长进行了 13 天的测量。两个品种的暗育苗前3个节间长度相似，证实了之前文献报道的进展9号在黑暗中具有高表型。在黑暗和红光下比较赤霉素 A20 (GA20) 的生物活性，赤霉素 A20 在正常情况下是无活性的。阿拉斯加的幼苗，无论生长条件如何，都对 GA20 做出反应。 Progress No. 9 的暗色生长的幼苗也对 GA20 有反应，但红光生长的幼苗则没有。赤霉素 A1 在两个品种中在黑暗和红光下均具有活性。 [13C3H]GA20 的代谢也已被研究。在阿拉斯加和 Progress No. 9 的黑暗生长芽中，[13C3H]GA20 转化为 [13C3H]GA1、[13C3H]GA8、[13C]GA29、其 2α-差向异构体和 [13C3H]GA29-分解代谢物。 [13C3H] 赤霉素 A1 是一种次要产物，似乎会快速转化，因此在某些饲料中仅检测到其代谢物 [13C3H]GA8。但结果确实表明，进步9号在黑暗中的高生长习性及其在黑暗中对GA20的反应能力可能与其3β-羟基化GA20生成GA1的能力有关。在红光下，两个品种的 [13C3H]GA20 总体代谢均降低。有一些证据表明，红光生长的阿拉斯加幼苗中可以发生[13C3H]GA20的3β-羟基化，但在红光生长的Progress中没有观察到[13C3H]GA20的3β-羟基化代谢物。因此，与其他矮化基因型一样，进展No. 9在红光下的矮化习性及其对GA20的无法反应可能与其无法将GA20 3β-羟基化为GA1有关。然而，两个品种中天然 GA 的鉴定和定量表明，红光生长的 Progress 确实含有天然 GA1。因此，红光生长的Progress No.9幼苗无法响应所施用的GA20和3β-羟基化物，可能是由于红光对GA的吸收和区隔的影响。
The stem growth in darkness or in continuous red light of two pea cultivars, Alaska (Le Le, tall) and Progress No. 9 (le le, dwarf), was measured for 13 d. The lengths of the first three internodes in dark-grown seedlings of the two cultivars were similar, substantiating previous literature reports that Progress No. 9 has a tall phenotype in the dark. The biological activity of gibberellin A20(GA20), which is normally inactive inle legeno-types, was compared in darkness and in red light. Alaska seedlings, regardless of growing conditions, responded to GA20. Dark-grown seedlings of Progress No. 9 also responded to GA20, although red-light-grown seedlings did not. Gibberellin A1was active in both cultivars, in both darkness and red light. The metabolism of [13C3H]GA20has also been studied. In dark-grown shoots of Alaska and Progress No. 9 [13C3H]GA20is converted to [13C3H]GA1, [13C3H]GA8, [13C]GA29, its 2α-epimer, and [13C3H]GA29-catabolite. [13C3H] Gibberellin A1was a minor product which appeared to be rapidly turned over, so that in some feeds only its metabolite, [13C3H]GA8, was detected. However results do indicate that the tall growth habit of Progress No. 9 in the dark, and its ability to respond to GA20in the dark may be related to its capacity to 3β-hydroxylate GA20to give GA1. In red light the overall metabolism of [13C3H]GA20was reduced in both cultivars. There is some evidence that 3β-hydroxylation of [13C3H]GA20can occur in red light-grown Alaska seedlings, but no 3β-hydroxylated metabolites of [13C3H]GA20were observed in red light-grown Progress. Thus the dwarf habit of Progress No. 9 in red light and its inability to respond to GA20may be related, as in other dwarf genotypes, to its inability to 3β-hydroxylate GA20to GA1. However identification and quantification of native GAs in both cultivars showed that red-light-grown Progress does contain native GA1. Thus the inability of red light-grown Progress No. 9 seedlings to respond to, and to 3β-hydroxylate, applied GA20may be due to an effect of red light on uptake and compartmentation of GAs.