Phenotypes of neural-crest-derived cells in vagal and sacral pathways
Phenotypes of neural-crest-derived cells in vagal and sacral pathways
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DOI：
10.1007/s00441-005-0047-6
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发表时间：
2006-01-01
影响因子：
3.6
通讯作者：
Young, HM
中科院分区：
文献类型：
--
作者：
Anderson, RB;Stewart, AL;Young, HM
肠神经元起源于迷走神经和骶神经水平的神经嵴细胞。为了研究迷走神经和骶神经通路中神经嵴衍生细胞的表型，我们使用了Sox 10、p75、Phox 2b和Hu的抗血清，以及绿色荧光蛋白表达受Ret启动子控制的转基因小鼠。Sox 10的表达之前，迷走神经细胞的移民，而p75的表达后不久，他们的移民。大多数嵴源性细胞迁移相邻体节1-4迁移沿着一条途径，后来由迷走神经。这些迷走神经细胞的亚群合并形成迷走神经节，而其他细胞继续向心脏和肠道迁移。合并成迷走神经节的细胞表现出与神经节近端和远端的迁移流中的细胞不同的表型。只有首先进入前肠的迷走神经细胞亚群表达Phox 2b或Ret。骶神经嵴细胞分化为盆神经节和后肠神经元。使用D β H-nlacZ小鼠检查骶神经嵴细胞的通路。骶细胞出现进入远端后肠胚胎第14.5天左右。很少有以前证明，但罕见的，神经元，存在于大肠中的Ret无效突变体，并推测产生从骶神经嵴表达一氧化氮合酶，不像他们的同行在Ret杂合子小鼠。
Enteric neurons arise from vagal and sacral level neural crest cells. To examine the phenotype of neural-crest-derived cells in vagal and sacral pathways, we used antisera to Sox10, p75, Phox2b, and Hu, and transgenic mice in which the expression of green fluorescent protein was under the control of the Ret promoter. Sox10 was expressed prior to the emigration of vagal cells, whereas p75 was expressed shortly after their emigration. Most crest-derived cells that emigrated adjacent to somites 1-4 migrated along a pathway that was later followed by the vagus nerve. A sub-population of these vagal cells coalesced to form vagal ganglia, whereas others continued their migration towards the heart and gut. Cells that coalesced into vagal ganglia showed a different phenotype from cells in the migratory streams proximal and distal to the ganglia. Only a sub-population of the vagal cells that first entered the foregut expressed Phox2b or Ret. Sacral neural crest cells gave rise to pelvic ganglia and some neurons in the hindgut. The pathways of sacral neural crest cells were examined by using D beta H-nlacZ mice. Sacral cells appeared to enter the distal hindgut around embryonic day 14.5. Very few of the previously demonstrated, but rare, neurons that were present in the large intestine of Ret null mutants and that presumably arose from the sacral neural crest expressed nitric oxide synthase, unlike their counterparts in Ret heterozygous mice.