OsHUB2 inhibits function of OsTrx1 in heading date in rice
OsHUB2 inhibits function of OsTrx1 in heading date in rice
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OsHUB2 抑制 OsTrx1 在水稻抽穗期的功能
DOI：
10.1111/tpj.15763
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Yong Ding
中科院分区：
文献类型：
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作者：
Zuntao Xu;Enze Li;Gan Xue;Cheng Zhang;Yachun Yang;Yong Ding
抽穗期是影响水稻产量和适应性的重要农艺性状之一。关于组蛋白泛素化和组蛋白甲基化在水稻抽穗期调控中的相互作用知之甚少。在此，我们证实组蛋白单核苷酸多态性1（OsHUB 1）和OsHUB 2通过Hd 1和Ehd 1途径参与抽穗期调控。OsHUB 1和OsHUB 2功能的缺失导致长日照和短日照光周期下的早抽穗。在长日照条件下，Hd 3a、RFT 1和Ehd 1的表达被诱导，而Hd 1、Ghd 7、OsCCA 1、OsGI、OsFKF 1和OsTOC 1的转录水平被降低，而在短日照条件下，RFT 1和Ehd 1的表达被诱导。OsHUB 2与OsTrx 1相互作用，抑制OsTrx 1基因的表达。OsHUB 2直接与Ehd 1结合，在Ehd 1处泛素化H2 B，hub 2 - 2和doshub 2 - 3突变体的H2 B泛素化水平降低。OsTrx 1在Ehd 1高度富集，并且Ehd 1的H3 K4 me 3水平在hub 2 - 2中上调。OsTrx 1在theoshub 2 - 2背景下的突变挽救了foshub 2 - 2的早抽穗表型。在shub 2 - 2突变体中Ehd 1H 3 K4 me 3水平和转录水平的增加在shub 2 - 2 ostrix 1 - 2双突变体中减弱。总之，我们的结果（i）揭示OsHUB 2抑制OsTrx 1和H3 K4 me 3水平在Ehd 1的功能;（ii）表明OsHUB 2介导的H2 B泛素化与H3 K4 me 3一起在水稻抽穗期调控中发挥关键作用。
Heading date is one of the most pivotal agronomic traits for rice (Oryza sativa) yield and adaptation. Little is known about the crosstalk between histone ubiquitination and histone methylation in rice heading date regulation. Here, we reportedHISTONE MONOUBIQUITINATION 1(OsHUB1) andOsHUB2are involved in heading date regulation via theHd1andEhd1pathway. Loss ofOsHUB1andOsHUB2function resulted in early heading under long‐day and short‐day photoperiods. The expression ofHd3a,RFT1, andEhd1was induced and the transcript levels ofHd1,Ghd7,OsCCA1,OsGI,OsFKF1, andOsTOC1were reduced under long‐day conditions, whereasRFT1andEhd1expression was induced inoshub2mutants under short‐day conditions. OsHUB2 interacted with OsTrx1 and repressed the gene expression ofOsTrx1. OsHUB2 directly bound toEhd1to ubiquitinate H2B atEhd1, and H2B ubiquitination levels were reduced inoshub2‐2andoshub2‐3mutants. OsTrx1 were highly enriched atEhd1, and H3K4me3 levels ofEhd1were upregulated inoshub2‐2. Mutations ofOsTrx1in theoshub2‐2background rescued the early‐heading phenotype ofoshub2‐2. The increases inEhd1H3K4me3 levels and transcript levels inoshub2‐2mutants were attenuated inoshub2‐2 ostrx1‐2double mutants. Together, our results (i) reveal that OsHUB2 represses the function of OsTrx1 and H3K4me3 levels atEhd1and (ii) suggest that OsHUB2‐mediated H2B ubiquitination plays critical roles together with H3K4me3 in rice heading date regulation.