Bioprospecting a glacial river in Iceland for bacterial biopolymer degraders
Bioprospecting a glacial river in Iceland for bacterial biopolymer degraders
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DOI：
10.1016/j.coldregions.2013.03.001
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发表时间：
2013-12
影响因子：
4.1
通讯作者：
J. Jóelsson;Heiða Friðjónsdóttir;O. Vilhelmsson
中科院分区：
文献类型：
--
作者：
J. Jóelsson;Heiða Friðjónsdóttir;O. Vilhelmsson
杰古尔萨·菲厄鲁姆冰川河发源于瓦特纳冰川冰盖，流经大片玄武岩火山灰沙漠，最终流入北冰洋，这里有许多迄今尚未开发的独特微生物栖息地。我们在沿河从源头到河口的 12 个采样点对河水、沉积物和其他生物生境进行了采样，收集了 382 种经过纯化和确认的可重复培养的耐冷细菌菌株。部分 16S rDNA 测序在 4 个细菌门中产生了 19 个属和 4 个非属特异性归属，其中假单胞菌和黄细菌尤其具有代表性。大部分分离株在 15°C 下产生胞外酶，包括淀粉酶、β-葡聚糖酶、纤维素酶、蛋白酶和漆酶。
The glacial river Jökulsá á Fjöllum, which originates in the Vatnajökull ice cap and flows through a large basaltic tephra desert on its way to discharge into the Arctic Ocean, presents a number of unique microbial habitats heretofore unexplored. We sampled river water, sediment and selected other biotopes at 12 sampling points along the river from source to mouth and generated a collection of 382 purified and confirmed reculturable psychrotrophic bacterial strains. Partial 16S rDNA sequencing yielded 19 genera and 4 non-genus specific assignments in 4 bacterial phyla, with pseudomonads and flavobacteria being particularly well represented. A large portion of the isolates produced extracellular enzymes at 15 °C, including amylase, betaglucanase, cellulase, protease and laccase.