Salvianic acid A protects L-02 cells against γ-irradiation-induced apoptosis via the scavenging of reactive oxygen species
Salvianic acid A protects L-02 cells against γ-irradiation-induced apoptosis via the scavenging of reactive oxygen species
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DOI：
10.1016/j.etap.2012.11.010
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发表时间：
2013-01-01
影响因子：
4.3
通讯作者：
Guo, Guozhen
中科院分区：
文献类型：
--
作者：
Guo, Juan;Zhang, Yanjun;Guo, Guozhen
丹酚酸A（SAA）是丹参的主要亲水活性成分，在我国长期以来被用于治疗肝脏和心脏病。在本研究中，我们研究了 SAA 对培养的人胚胎肝 L-02 细胞中伽马辐射诱导的细胞凋亡的辐射防护作用。结果表明，SAA显着抑制γ射线诱导的细胞凋亡，减少DNA损伤，提高L-02细胞的细胞内抗氧化能力。 SAA通过减少活性氧的产生、抑制线粒体细胞色素C的释放、阻断caspase-3的激活、下调Bax和P53的表达、上调Bcl-2的表达来发挥辐射防护作用。这表明SAA预处理抑制了caspase依赖性线粒体凋亡途径。与雌三醇暴露相比，SAA预处理的放射防护作用还体现在存活率的提高、肝脏中抗氧化酶水平的维持、氧化应激的抑制以及相对较低的肝肾毒性。总之，SAA 可能是一种有效的放射防护剂，可抵抗伽马射线诱导的 L-02 细胞凋亡和小鼠损伤，SAA 的抗氧化效力可能与观察到的有益放射防护作用相关。 (C) 2012 Elsevier B.V. 保留所有权利。
Salvianic acid A (SAA) is the main hydrophilic active ingredient of Salvia miltiorrhiza bunge, which has long been used to treat liver and heart disease in China. In the present study, we investigated the radioprotective effects of SAA against gamma-radiation-induced apoptosis in cultured human embryo liver L-02 cells. The results demonstrated that SAA markedly inhibited gamma-radiation induced apoptosis, decreased DNA damage, and increased the intracel-lular antioxidative ability of the L-02 cells. SAA exhibited radioprotection by decreasing the generation of reactive oxygen species, inhibiting the release of mitochondrial cytochrome C, blocking the activation of caspase-3, and down regulating the expression of Bax and P53 and up regulating the expression of Bcl-2. This indicated that SAA pretreatment inhibited the caspase-dependent mitochondria apoptosis pathway. The radioprotection of the SAA pretreatment was also evidenced by an increased survival ratio, maintaining the antioxidant enzyme levels in the liver, inhibition of oxidative stress, and relative low liver and renal toxicity compared with estriol exposure. In conclusion, SAA may be an effective radioprotector against gamma-radiation induced apoptosis in L-02 cells and damage in mice, the antioxidant potency of SAA might be correlated with the beneficial radioprotectant effects observed. (C) 2012 Elsevier B.V. All rights reserved.