Identification and characterisation of mRif1: A mouse telomere-associated protein highly expressed in germ cells and embryo-derived pluripotent stem cells
Identification and characterisation of mRif1: A mouse telomere-associated protein highly expressed in germ cells and embryo-derived pluripotent stem cells
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DOI：
10.1002/dvdy.10471
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发表时间：
2004-04-01
影响因子：
2.5
通讯作者：
McLaren, A
中科院分区：
文献类型：
--
作者：
Adams, IR;McLaren, A
我们已经确定了一个小鼠直系同源的酵母Rif 1家族的端粒相关蛋白的基础上，其在原始生殖细胞和胚胎来源的多能干细胞系的高表达。mRif 1也在小鼠发育早期的全能和多能细胞中以及成年小鼠的雄性和雌性生殖细胞中高度表达。mRif 1的表达在衍生过程中被诱导。胚胎干细胞，并在体外胚胎干细胞分化时迅速下调。此外，我们表明，mRif 1物理相互作用的端粒相关蛋白mTrf 2，并可以交联到小鼠胚胎干细胞中的端粒重复DNA。mRif 1可能参与维持端粒长度或多能性的生殖细胞和早期小鼠发育。(C)2004 Wiley-Liss，Inc.
We have identified a mouse ortholog of the yeast Rif1 family of telomere-associated proteins on the basis of its high expression in primordial germ cells and embryo-derived pluripotent stem cell lines. mRif1 is also highly expressed in totipotent and pluripotent cells during early mouse development, and in male and female germ cells in adult mice. mRif1 expression is induced during derivation of. embryonic stem cells and is rapidly down-regulated upon differentiation of embryonic stem cells in vitro. Furthermore, we show that mRif1 physically interacts with the telomere-associated protein mTrf2 and can be cross-linked to telomeric repeat DNA in mouse embryonic stem cells. mRif1 may be involved in the maintenance of telomere length or pluripotency in the germline and during early mouse development. (C) 2004 Wiley-Liss, Inc.