Ancient mitochondrial DNA reveals the origin of Sus scrofa from Rebun Island, Japan
Ancient mitochondrial DNA reveals the origin of Sus scrofa from Rebun Island, Japan
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DOI：
10.1007/s002390010156
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发表时间：
2001-03-01
影响因子：
3.9
通讯作者：
Ushiro, H
中科院分区：
文献类型：
--
作者：
Watanabe, T;Ishiguro, N;Ushiro, H
歌舞伎A遗址（公元5 ~ 8世纪）鄂霍次克文化区位于日本北海道西北偏北的一个靠近海岸的小岛礼文岛上。在Kabukai A遗址的五个文化层中发现了Sus scrofa的标本，称为萨哈林猪。古代：从42块库页岛猪骨遗骸中提取DNA。39个核苷酸序列的574-bp的线粒体DNA控制区，估计已经起源于至少21个个体，扩增和遗传学分析。通过简约网络分析，将该位点的9种单倍型（A1，A2，A3，B1，B2，C1，C2，D1，D2）分为4个单倍型群（A，B，C，D）。对9个古代和55个现代单倍型的系统发育分析表明，Kabukai A地点的库页岛猪群属于两个不同的类群;单倍型组A和B形成仅由它们自身组成的簇，单倍型组C和D属于独特地分布在日本西部的日本野猪的两个遗传组之一的簇，包括一只东北蒙古野猪。通过对三个考古遗址的单倍型分布及其在反映Kabukai A遗址文化时期的五个层次之间的历史变迁分析，表明萨哈林猪群是从欧亚大陆东北部的萨哈林岛和阿穆尔河流域引入的，并受到了一定的文化影响。
The Kabukai A site (5 to 8C A.D.) of the Okhotsk cultural area is on Rebun Island, a small island near the coast, north-northwest of Hokkaido, Japan. Specimens of Sus scrofa, called the Sakhalin pig, were discovered in five cultural layers at the Kabukai A site. Ancient: DNA was extracted from the remains of 42 Sakhalin pig bones. Thirty-nine nucleotide sequences of the 574-bp mitochondrial DNA control region, estimated to have originated from at least 21 individuals, were amplified and analyzed phylogenetically. Nine distinct haplotypes (A1, A2, A3, B1, B2, C1, C2, D1, and D2) from this site were classified into four haplotype groups (A, B, C, and D) by parsimonious network analysis. Phylogenetic analysis of 9 ancient and 55 modern haplotypes indicated that the population of Sakhalin pigs at the Kabukai A site belonged to two distinct clusters; haplotype groups A and B formed a cluster comprised only of themselves, and haplotype groups C and D belonged to the cluster of one of the two genetic groups of Japanese wild boars uniquely distributed in the western part of Japan, including one northeast Mongolian wild boar. Analysis of the haplotype distribution among three archaeological sites and their historical transitions among the five layers reflecting the cultural periods at the Kabukai A site suggests that the Sakhalin pig populations were introduced from Sakhalin island and the Amur River basin in the northeastern Eurasian continent together with some cultural influences.