Sweet action: The dynamics of O-GlcNAcylation during meiosis in mouse oocytes.
Sweet action: The dynamics of O-GlcNAcylation during meiosis in mouse oocytes.
复制标题
DOI：
10.1002/mrd.22577
复制
发表时间：
2015-12
影响因子：
2.5
通讯作者：
Duncan FE
中科院分区：
文献类型：
--
作者：
Slawson C;Duncan FE
翻译后修饰调节有丝分裂和减数分裂。例如，O-GlcNAc酰化--在丝氨酸或苏氨酸残基上添加一个N-乙酰葡糖胺（O-GlcNAc）--是有丝分裂纺锤体形成和染色体分离所必需的;在这里，我们证明了在小鼠卵母细胞减数分裂过程中O-GlcNAc酰化发生。O-GlcNAc转移酶（OGT）和O-GlcNAc酶（OGA）分别添加和去除这种修饰，在减数分裂期间表达并具有活性。用O-GlcNAc（绿色）修饰的蛋白质在前期I（顶部细胞）集中在核膜处，并且在中期II（底部细胞）可以在整个细胞质中发现，富集紧邻浓缩的染色体。OGT（黄色）在前期I分布在整个卵母细胞中，但随后集中在中期II纺锤体中。相反，OGA（青色）在整个减数分裂过程中主要富集在皮层。OGT和OGA的这些不同的空间分布表明O-GlcNAc化维持在卵母细胞的离散区域-例如减数分裂纺锤体。研究O-GlcNAc，OGT和OGA在减数分裂成熟过程中的精确功能将为翻译后修饰对配子质量的影响提供新的见解。
Post-translational modifications regulate mitosis and meiosis. For example, O-GlcNAcylation –the addition of a single N acetyl-glucosamine (O-GlcNAc) to serine or threonine residues– is required for mitotic spindle formation and chromosome segregation; here we show that O-GlcNAcylation occurs in mouse oocytes during meiosis. O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA), which respectively adds and removes this modification, are expressed and active during meiosis. Proteins modified with O-GlcNAc (green) are concentrated at the nuclear envelope at prophase I (top cells) and can be found throughout the cytoplasm at metaphase II (bottom cells), with an enrichment immediately adjacent to the condensed chromosomes. OGT (yellow) is distributed throughout the oocyte at prophase I, but is later concentrated at the metaphase-II spindle. In contrast, OGA (cyan) is enriched primarily at the cortex throughout meiosis. These distinct spatial distributions of OGT and OGA suggest that O-GlcNAcylation is maintained at discrete regions of the oocyte – e.g. the meiotic spindle. Studies investigating the precise function of O-GlcNAc, OGT, and OGA during meiotic maturation will provide new insights into the impact of post-translational modifications on gamete quality.