Distribution of Pseudomonas fluorescens and Aeromonas hydrophila Bacteria in a Recirculating Aquaculture System during Farming of European Grayling (Thymallus thymallus L.) Broodstock
Distribution of Pseudomonas fluorescens and Aeromonas hydrophila Bacteria in a Recirculating Aquaculture System during Farming of European Grayling (Thymallus thymallus L.) Broodstock
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DOI：
10.3390/w11020376
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发表时间：
2019-02-01
期刊：
影响因子：
3.4
通讯作者：
Glinska-Lewczuk, Katarzyna
中科院分区：
文献类型：
--
作者：
Golas, Iwona;Szmyt, Mariusz;Glinska-Lewczuk, Katarzyna
荧光假单胞菌和嗜水气单胞菌是自然存在于水环境和健康鱼类肠道菌群中的条件致病菌。这两个物种都可能对对水污染高度敏感的鱼类构成严重威胁。这项研究的目的是确定投喂鱼饲料的量和鱼的生物量对Ps的分布和丰度的影响程度。欧洲灰鱼(Thymallus Thyallus L.)养殖过程中循环水产养殖系统(RAS)中的荧光菌和嗜水气单胞菌亲鱼。分别从进水口、两个养殖池和出水口采集了68个水样和17个饲料样品进行了分析。细菌种群分析采用依赖于培养的方法和分子方法(荧光原位杂交，FISH)，分别检测可培养菌株和活的但不可培养的菌株。鱼类生物量、饲料和16个水质参数(温度、pH、溶解氧浓度、氧饱和度、5日生化需氧量(BOD5)、总磷、总有机磷和总氮、正磷酸盐、总氮、亚硝酸盐和硝态氮、氨氮、氨氮、总悬浮物和总有机碳)是解释因素。来自流入、养殖池和流出的样本中的细菌丰度有统计学意义的差异(rm-ANOVA，p 0.05)。来自RAS的水样大量被非可培养的P。荧光菌和嗜水气单胞菌。饲料不是细菌的来源，但冗余分析(RDA)显示，饲料、鱼的生物量、BOD5、总悬浮物和总有机碳在两个P中呈正相关。荧光菌和嗜水气单胞菌。这些参数还影响了潜在致病细菌种群的分布，并导致了RAS水的细菌污染。我们的结果对于帮助补充野生种群和重建自然水环境中受威胁物种种群的水产养殖特别有价值。
Pseudomonas fluorescens and Aeromonas hydrophila bacteria are opportunistic pathogens that occur naturally in the aquatic environment and in the gut flora of healthy fish. Both species can pose a serious threat for fish that are highly sensitive to water pollution. The aim of this study was to determine the extent to which the amount of administered fish feed and fish biomass affect the distribution and abundance of Ps. fluorescens and A. hydrophila bacteria in a recirculating aquaculture system (RAS) during farming of European grayling (Thymallus thymallus L.) broodstock. A total of 68 water samples from the inflow, two rearing tanks and the outflow as well as 17 feed samples were collected and analyzed separately. Bacterial populations were analyzed by the culture-dependent method and a molecular method (fluorescence in situ hybridization, FISH) to detect culturable strains and viable but non-culturable strains, respectively. Fish biomass, feed and 16 water quality parameters (temperature, pH, concentration of dissolved oxygen, oxygen saturation, five-day biochemical oxygen demand (BOD5), total phosphorus, total organic phosphorus and nitrogen, orthophosphates, total nitrogen, nitrite and nitrate nitrogen, ammonia nitrogen, ammonium nitrogen, total suspended solids, and total organic carbon) were the explanatory factors. Statistically significant differences (RM-ANOVA, p 0.05) were stated in bacterial abundance in samples from the inflow, rearing tanks and the outflow. Water samples from the RAS were abundantly colonized by non-culturable Ps. fluorescens and A. hydrophila bacteria. Feed was not a source of bacteria, but a redundancy analysis (RDA) revealed that the amount of feed, fish biomass, BOD5, and total suspended solids and total organic carbon were positively correlated in both Ps. fluorescens and A. hydrophila. These parameters also influenced the distribution of both potentially pathogenic bacterial populations and contributed to the bacterial contamination of water in the RAS. Our results are particularly valuable for aquacultures that help to replenish wild stocks and rebuild populations of threatened species in natural aquatic environments.