Cytoplasmic HuR expression correlates with epithelial cancer cell but not with stromal cell cyclooxygenase-2 expression in mucinous ovarian carcinoma
Cytoplasmic HuR expression correlates with epithelial cancer cell but not with stromal cell cyclooxygenase-2 expression in mucinous ovarian carcinoma
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DOI：
10.1016/j.ygyno.2005.04.047
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发表时间：
2005-10-01
影响因子：
4.7
通讯作者：
Ristimäki, A
中科院分区：
文献类型：
--
作者：
Erkinheimo, TL;Sivula, A;Ristimäki, A
环氧合酶-2（考克斯-2）的表达与几种类型的肿瘤预后不良有关。HuR是一种mRNA稳定性蛋白，调节考克斯-2的表达。采用免疫组化法检测64例卵巢粘液癌组织中考克斯-2和HuR的表达。在粘液性肿瘤中，39%（22/56）的上皮癌细胞和24%（13/55）的间质细胞中发现高表达考克斯-2蛋白。考克斯-2在癌细胞中的表达与高级别相关（P = 0.0285），而间质考克斯-2的表达与临床指标无关。在粘液性肿瘤中，47%（27/57）的癌细胞和7%（4/56）的间质细胞胞浆HuR蛋白表达阳性，且HuR蛋白表达与考克斯-2在癌细胞中的表达相关（P = 0.0162），而与间质中的表达无关。我们的研究结果支持这样的假设，即细胞质HuR与卵巢癌中考克斯-2的表达有关，但其作用仅限于转化的上皮癌细胞。(c)2005年爱思唯尔公司All rights reserved.
Cyclooxygenase-2 (COX-2) expression has been found to associate with poor prognosis in several types of carcinomas. HuR is an mRNA stability protein and it regulates the expression of COX-2.Objectives and methods. We analyzed the expression of COX-2 and HuR in 64 mucinous ovarian carcinoma specimens by immunohistochemistry.Results. In mucinous tumors, high COX-2 protein expression was found in epithelial cancer cells in 39% (22/56) and in stromal cells in 24% (13/55) of the specimens. The expression of COX-2 in cancer cells correlated with high grade (P = 0.0285), but stromal COX-2 expression had no correlation with any clinical parameter tested. Cytoplasmic HuR protein expression was observed in cancer cells in 47% (27/57) and in stromal cells in 7% (4/56) of the mucinous tumors, and it correlated with COX-2 expression in the cancer cells (P = 0.0162) but not in the stroma.Conclusion. Our results support the hypothesis that cytoplasmic HuR is connected to COX-2 expression in ovarian carcinoma, but that its role is restricted to the transformed epithelial cancer cells. (c) 2005 Elsevier Inc. All rights reserved.