The cardiac gap junction protein (Mr 47,000) has a tissue-specific cytoplasmic domain of Mr 17,000 at its carboxy-terminus.
The cardiac gap junction protein (Mr 47,000) has a tissue-specific cytoplasmic domain of Mr 17,000 at its carboxy-terminus.
复制标题
心脏间隙连接蛋白 (Mr 47,000) 在其羧基末端具有组织特异性细胞质结构域 Mr 17,000。
DOI：
10.1016/0006-291x(87)90475-x
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发表时间：
1987
影响因子：
3.1
通讯作者：
Revel,JP
中科院分区：
文献类型：
--
作者：
Manjunath,CK;Nicholson,BJ;Teplow,D;Hood,L;Page,E;Revel,JP
心脏间隙连接蛋白亚基的分子量，直到最近，被认为是约Mr 28，000 - 30，000，类似于其他先前表征的间隙连接蛋白。一种约Mr 44，000 - 47，000的较大多肽，在分离过程中经历蛋白水解，最近被提出作为体内心脏连接蛋白的形式。我们在这里表明，该实体具有相同的氨基末端序列作为先前表征的M r 29，000 - 30，000组件。因此，心脏连接蛋白在其羧基末端有一个Mr 17，000的胞质结构域;这个结构域在肝脏蛋白中不存在。这些观察结果提供了进一步的证据，间隙连接蛋白形成一个高度多样化的家庭。
The molecular weight of the heart gap junctional protein subunit was, until recently, believed to be about M r 28,000–30,000, similar to that of other previously characterized gap junctional proteins. A larger polypeptide of about M r 44,000–47,000, which undergoes proteolysis during isolation, has recently been proposed as the form of the heart junction protein in vivo. We show here that this entity has the same aminoterminal sequence as the previously characterized M r 29,000–30,000 component. Thus, the cardiac junctional protein has, at its carboxy-terminus, a cytoplasmic domain of M r 17,000; this domain is absent in the liver protein. These observations provide further evidence that gap junction proteins form a highly diversified family.