Ammonia Inhibition of Methanogenesis and Identification of Process Indicators during Anaerobic Digestion
Ammonia Inhibition of Methanogenesis and Identification of Process Indicators during Anaerobic Digestion
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DOI：
10.1089/ees.2007.0282
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发表时间：
2008-09
影响因子：
1.8
通讯作者：
R. Nakakubo;H. Møller;A. M. Nielsen;J. Matsuda
中科院分区：
文献类型：
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作者：
R. Nakakubo;H. Møller;A. M. Nielsen;J. Matsuda
在连续搅拌釜反应器实验中，使用猪粪与从猪粪中分离的固体部分共消化（质量混合比为 40:60），有机负荷率为 9.4 gVS/消化器/天，高温温度为 51°C，我们通过使用氯化铵脉冲研究了氨对甲烷生成的急性抑制作用，并评估了挥发性脂肪酸 (VFA) 是否可以用作过程指示剂。尽管消化过程稳定并且产甲烷菌适应了高氨浓度，但与固体部分的共消化似乎导致轻微的氨抑制作用。我们发现总氨浓度和甲烷气体产量之间存在很强的负相关性（R 2 = 0.91）。然而，游离氨浓度并不能显着反映急性氨抑制作用。与氨浓度为 4.6 gNH4–N/l 且无脉冲氯化铵的对照反应器相比，甲烷气体产率（以负荷计算）降低了 50%。
In continuously stirred tank reactor experiments using pig manure codigested with solid fractions separated from pig manure (a mass mixture ratio of 40:60) with an organic loading rate of 9.4 gVS/ldigester/day at a thermophilic temperature of 51°C, we investigated acute inhibitory effects of ammonia on methanogenesis by using pulses of ammonium chloride, and assessed whether volatile fatty acids (VFAs) could be used as process indicators. Codigestion with the solid fractions seemed to result in a slight ammonia inhibition effect, though the digestion process was stable and the methanogens were adapted to a high ammonia concentration. We found a strong negative correlation (R 2 = 0.91) between total ammonia concentration and methane gas yield. However, free ammonia concentration did not reflect the acute ammonia inhibition significantly. Compared to a control reactor which was at an ammonia concentration of 4.6 gNH4–N/l, without pulsing ammonium chloride, a 50% decrease in methane gas yield in terms of loa...