Metabolic control and its analysis
Metabolic control and its analysis
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代谢控制及其分析
DOI：
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发表时间：
1985
期刊：
影响因子：
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通讯作者：
H. Sauro
中科院分区：
文献类型：
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作者：
D. Fell;H. Sauro
代谢控制分析中的现有定理已被采用并嵌入到一个简单的矩阵代数程序中，用于根据酶的动力学特性（其弹性）计算代谢途径中酶的通量控制系数（以前称为灵敏度）。新的定理支配的分支路径和基板周期的通量控制系数已被推导出允许的程序被应用到复杂的路径配置。在方程中使用的弹性项的修改已在理论上证明，使该方法仍然有效的途径与保守的代谢物（例如，腺嘌呤核苷酸池或中间体的催化循环，如三羧酸循环）或池的代谢物保持非常接近平衡非常快速的反应。
使用这些定理和关系产生的矩阵方程可以用代数或数值方法求解。代数的解决方案已被用来确定的因素负责的程度放大的通量控制系数的底物循环，并表明它是可能的，以获得一组酶的弹性的表达式。
Existing theorems from the analysis of metabolic control have been taken and embedded in a simple matrix algebra procedure for calculating the flux control coefficients of enzymes (formerly known as sensitivities) in a metabolic pathway from their kinetic properties (their elasticities). New theorems governing the flux control coefficients of branched pathways and substrate cycles have been derived to allow the procedure to be applied to complex pathway configurations. Modifications to the elasticity terms used in the equations have been theoretically justified so that the method remains valid for pathways with conserved metabolites (for example, the adenine nucleotide pool or the intermediates of a catalytic cycle such as the tricarboxylic acid cycle) or with pools of metabolites kept very near to equilibrium by very rapid reactions.
The matrix equations generated using these theorems and relationships may be solved algebraically or numerically. Algebraic solutions have been used to determine the factors responsible for the degree of amplification of flux control coefficients by substrate cycles and to show that it is possible to derive expressions for the elasticities of a group of enzymes.