The Plasmodium falciparum protein Pfg27 is dispensable for gametocyte and gamete production, but contributes to cell integrity during gametocytogenesis
The Plasmodium falciparum protein Pfg27 is dispensable for gametocyte and gamete production, but contributes to cell integrity during gametocytogenesis
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DOI：
10.1111/j.1365-2958.2009.06762.x
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发表时间：
2009-07-01
影响因子：
3.6
通讯作者：
Alano, Pietro
中科院分区：
文献类型：
--
作者：
Olivieri, Anna;Camarda, Grazia;Alano, Pietro
在人类疟疾寄生虫恶性疟原虫中，配子体成熟的过程比其他疟疾物种明显更长，伴随着2-300种性阶段特异性蛋白的表达。到目前为止，对它们的几个编码基因的破坏表明，只有在性分化开始时产生的丰富的蛋白Pfg 27对配子体的产生是必不可少的。与先前所描述的相反，在此我们表明恶性疟原虫pfg 27破坏株系能够经历配子母细胞成熟的所有阶段，并且能够成熟为配子。Pfg 27缺陷的配子母细胞的一部分，但是，在内部和细胞外的膜室，如寄生虫空泡衍生的囊泡在红细胞的细胞质中，大的细胞内空泡和不连续性的积累，在其三层细胞膜的明显异常。这项工作修订了目前对Pfg 27作用的认识，表明该蛋白质不是寄生虫进入性分化所必需的，并表明它在恶性疟原虫独特的长配子体发生中参与维持细胞完整性。
In the human malaria parasite Plasmodium falciparum, gametocyte maturation is a process remarkably longer than in other malaria species, accompanied by expression of 2-300 sexual stage-specific proteins. Disruption of several of their encoding genes so far showed that only the abundant protein Pfg27, produced at the onset of sexual differentiation, is essential for gametocyte production. In contrast with what has been previously described, here we show that P. falciparum pfg27 disruptant lines are able to undergo all stages of gametocyte maturation, and are able to mature into gametes. A fraction of Pfg27-defective gametocytes show, however, distinct abnormalities in intra- and extracellular membranous compartments, such as accumulation of parasitophorous vacuole-derived vesicles in the erythrocyte cytoplasm, large intracellular vacuoles and discontinuities in their trilaminar cell membrane. This work revises current knowledge on the role of Pfg27, indicating that the protein is not required for parasite entry into sexual differentiation, and suggesting that it is instead involved in maintaining cell integrity in the uniquely long gametocytogenesis of P. falciparum.