SUCROSE PHOSPHATE SYNTHASE AND SUCROSE ACCUMULATION AT LOW-TEMPERATURE
SUCROSE PHOSPHATE SYNTHASE AND SUCROSE ACCUMULATION AT LOW-TEMPERATURE
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DOI：
10.1104/pp.100.1.502
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发表时间：
1992-09-01
期刊：
影响因子：
7.4
通讯作者：
HUBER, SC
中科院分区：
文献类型：
--
作者：
GUY, CL;HUBER, JLA;HUBER, SC
研究了生长温度对菠菜（Spinacia oleracea）叶片组织中游离糖和蔗糖磷酸合成酶含量及活性的影响。当植物生长在25摄氏度3周，然后转移到一个恒定的5摄氏度，蔗糖，葡萄糖和果糖积累到高水平，在14天的时间。黎明前的糖水平比低温处理开始时的水平增加了14到20倍。在暴露于5 ℃之前、期间和之后，蔗糖是最丰富的游离糖。低温处理显著提高了叶片蔗糖磷酸合成酶的活性，而蔗糖合成酶和转化酶的活性则没有显著提高。蔗糖磷酸合成酶亚基的合成增加期间和低温暴露后，并在亚基的稳态水平增加。蔗糖及其主要生物合成酶，蔗糖磷酸合成酶的增加，讨论了有关调节代谢低不冻温度和冷冻胁迫的耐受性。
The influence of growth temperature on the free sugar and sucrose phosphate synthase content and activity of spinach (Spinacia oleracea) leaf tissue was studied. When plants were grown at 25-degrees-C for 3 weeks and then transferred to a constant 5-degrees-C, sucrose, glucose, and fructose accumulated to high levels during a 14-d period. Predawn sugar levels increased from 14- to 20-fold over the levels present at the outset of the low-temperature treatment. Sucrose was the most abundant free sugar before, during, and after exposure to 5-degrees-C. Leaf sucrose phosphate synthase activity was significantly increased by the low-temperature treatment, whereas sucrose synthase and invertases were not. Synthesis of the sucrose phosphate synthase subunit was increased during and after low-temperature exposure and paralleled an increase in the steady-state level of the subunit. The increases in sucrose and its primary biosynthetic enzyme, sucrose phosphate synthase, are discussed in relation to adjustment of metabolism to low nonfreezing temperature and freezing stress-tolerance.