Uptake and Toxicity of Respirable Carbon-Rich Uranium-Bearing Particles: Insights into the Role of Particulates in Uranium Toxicity.
Uptake and Toxicity of Respirable Carbon-Rich Uranium-Bearing Particles: Insights into the Role of Particulates in Uranium Toxicity.
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DOI：
10.1021/acs.est.1c01205
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发表时间：
2021-07-20
影响因子：
11.4
通讯作者：
Cerrato JM
中科院分区：
文献类型：
--
作者：
El Hayek E;Medina S;Guo J;Noureddine A;Zychowski KE;Hunter R;Velasco CA;Wiesse M;Maestas-Olguin A;Brinker CJ;Brearley A;Spilde M;Howard T;Lauer FT;Herbert G;Ali AM;Burchiel S;Campen MJ;Cerrato JM
颗粒物（PM）对居住在铀（U）矿区附近的社区构成环境健康风险。然而，颗粒形式的U对其细胞毒性的作用仍然知之甚少。在这里，我们研究了富碳铀颗粒的细胞摄取和毒性，作为一个模型有机颗粒含有铀酰柠檬酸在一系列环境相关的浓度的U（0-445 μM）。富碳含铀颗粒对人上皮细胞（A549）的细胞毒性具有U剂量依赖性。可溶性U剂量未检测到细胞毒性效应。在100 μM铀浓度下，宽粒径分布（<10 μm）的富碳铀颗粒比窄粒径分布（<1 μm）的富碳铀颗粒的细胞铀摄取量高2.7倍。TEM-EDS分析确定了富含C的U-轴承颗粒簇的细胞内易位。富含C的U-轴承颗粒的积累诱导DNA损伤和细胞毒性，如分别通过组蛋白H2 AX的磷酸化增加和细胞死亡所指示的。这些研究结果揭示了环境相关的异质粒度分布下的U的颗粒形式的毒性。我们的研究开辟了新的途径，未来的调查对健康的影响所造成的环境暴露的微粒形式的U矿附近的网站。
Particulate matter (PM) presents an environmental health risk for communities residing close to uranium (U) mine sites. However, the role of the particulate form of U on its cellular toxicity is still poorly understood. Here, we investigated the cellular uptake and toxicity of C-rich U-bearing particles as a model organic particulate containing uranyl citrate over a range of environmentally relevant concentrations of U (0–445 μM). The cytotoxicity of C-rich U-bearing particles in human epithelial cells (A549) was U-dose-dependent. No cytotoxic effects were detected with soluble U doses. Carbon-rich U-bearing particles with a wide size distribution (<10 μm) presented 2.7 times higher U uptake into cells than the particles with a narrow size distribution (<1 μm) at 100 μM U concentration. TEM-EDS analysis identified the intracellular translocation of clusters of C-rich U-bearing particles. The accumulation of C-rich U-bearing particles induced DNA damage and cytotoxicity as indicated by the increased phosphorylation of the histone H2AX and cell death, respectively. These findings reveal the toxicity of the particulate form of U under environmentally relevant heterogeneous size distributions. Our study opens new avenues for future investigations on the health impacts resulting from environmental exposures to the particulate form of U near mine sites.
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影响因子：
4.3
作者：
Buerglova, Kristyna;Noureddine, Achraf;Man, Michel Wong Chi
通讯作者：
Man, Michel Wong Chi
影响因子：
46.2
作者：
Behzadi S;Serpooshan V;Tao W;Hamaly MA;Alkawareek MY;Dreaden EC;Brown D;Alkilany AM;Farokhzad OC;Mahmoudi M
通讯作者：
Mahmoudi M
影响因子：
3.8
作者：
Ahamed, Maqusood;Karns, Michael;Hong, Yiling
通讯作者：
Hong, Yiling
影响因子：
11.4
作者：
Gil-Loaiza J;Field JP;White SA;Csavina J;Felix O;Betterton EA;Sáez AE;Maier RM
通讯作者：
Maier RM
影响因子：
3.9
作者：
Deditius, Artur P.;Utsunomiya, Satoshi;Ewing, Rodney C.
通讯作者：
Ewing, Rodney C.