Passive immunization of channel catfish (Ictalurus punctatus) against the ciliated protozoan parasite Ichthyophthirius multifiliis by use of murine monoclonal antibodies
Passive immunization of channel catfish (Ictalurus punctatus) against the ciliated protozoan parasite Ichthyophthirius multifiliis by use of murine monoclonal antibodies
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DOI：
10.1128/iai.64.10.4085-4090.1996
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发表时间：
1996-10-01
影响因子：
3.1
通讯作者：
Dickerson, H
中科院分区：
文献类型：
--
作者：
Lin, TL;Clark, TG;Dickerson, H
鱼类对纤毛原生动物寄生虫多丝小瓜虫（Ichthyophthirius multifiliis）亚致死感染后获得免疫，免疫应答包括针对一类被称为固定抗原的丰富的表面膜蛋白的体液抗体的制备针对这些蛋白质的抗体在体外杀死寄生虫，表明i-抗原在保护性免疫中的潜在作用。为了验证这一假设，使用固定的鼠单克隆抗体（MAb），用未受感染的癍点叉尾（Ictaluruspunctatus）进行被动免疫实验。在腹膜内注射20 - 200 μ g MAb后，鱼被完全保护免于致死性攻击。尽管鱼在较低剂量下死于感染，但用少至2 μ g的抗体观察到缓解作用，在接种后不同时间对动物进行攻击的实验中，发现寄生虫负荷和血清固定活性之间呈反比关系。在7种具有保护作用的单克隆抗体中，均为免疫球蛋白G类抗体。唯一未能保护的固定化单克隆抗体是免疫球蛋白M抗体，该抗体通过酶联免疫吸附测定法测定在表面粘膜中不存在。讨论了鱼类的多丝状体和对表面病原体的免疫。
Fish acquire immunity against the ciliated protozoan parasite Ichthyophthirius multifiliis following sublethal infection, The immune response includes the elaboration of humoral antibodies against a class of abundant surface membrane proteins referred to as immobilization antigens (i-antigens), Antibodies against these proteins immobilize the parasite in vitro, suggesting a potential role for the i-antigens in protective immunity, To test this hypothesis, passive immunization experiments were carried out with naive channel catfish, Ictalurus punctatus, using immobilizing murine monoclonal antibodies (MAbs), Fish were completely protected against lethal challenge following intraperitoneal injection of 20 to 200 mu g of MAb, Although fish succumbed to infection at lower doses, palliative effects were observed with as little as 2 mu g of antibody, In experiments in which animals were challenged at various times following inoculation, an inverse relationship between parasite load and serum immobilizing activity was seen, Of seven MAbs which conferred protection, all were immunoglobulin G class antibodies. The only immobilizing MAb that failed to protect was an immunoglobulin M antibody that was absent from surface mucosa as determined by enzyme-linked immunosorbent assay, The implications of these findings for the development of a vaccine against I. multifiliis and immunity against surface pathogens of fish are discussed.